Abstract
Exclusion of the α-exon by alternative RNA splicing of the fibroblast growth factor receptor 1 (FGFR1) primary transcript leads to the production of FGFR1β. Glial cell transformation is associated with a progressive increase in FGFR1β expression that coincides with a dramatic increase in the expression of the splicing factor polypyrimidine tract-binding protein (PTB). Cell-specific overexpression of PTB increased α-exon skipping, and a reduction in PTB increased α-exon inclusion. Targeted disruption of PTB interaction with FGFR1 precursor RNA in vivo by an antisense oligonucleotide also increased α-exon inclusion. These results suggest that PTB plays a direct role in α-exon splicing.
Original language | English (US) |
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Pages (from-to) | 6154-6157 |
Number of pages | 4 |
Journal | Cancer Research |
Volume | 63 |
Issue number | 19 |
State | Published - Oct 1 2003 |
ASJC Scopus subject areas
- Oncology
- Cancer Research