TY - JOUR
T1 - Preclinical anatomical, molecular, and functional imaging of the lung with multiple modalities
AU - Gammon, Seth T.
AU - Foje, Nathan
AU - Brewer, Elizabeth M.
AU - Owers, Elizabeth
AU - Downs, Charles A.
AU - Budde, Matthew D.
AU - Leevy, W. Matthew
AU - Helms, My N.
PY - 2014
Y1 - 2014
N2 - In vivo imaging is an important tool for preclinical studies of lung function and disease. The widespread availability of multimodal animal imaging systems and the rapid rate of diagnostic contrast agent development have empowered researchers to noninvasively study lung function and pulmonary disorders. Investigators can identify, track, and quantify biological processes over time. In this review, we highlight the fundamental principles of bioluminescence, fluorescence, planar X-ray, X-ray computed tomography, magnetic resonance imaging, and nuclear imaging modalities (such as positron emission tomography and single photon emission computed tomography that have been successfully employed for the study of lung function and pulmonary disorders in a preclinical setting. The major principles, benefits, and applications of each imaging modality and technology are reviewed. Limitations and the future prospective of multimodal imaging in pulmonary physiology are also discussed. In vivo imaging bridges molecular biological studies, drug design and discovery, and the imaging field with modern medical practice, and, as such, will continue to be a mainstay in biomedical research.
AB - In vivo imaging is an important tool for preclinical studies of lung function and disease. The widespread availability of multimodal animal imaging systems and the rapid rate of diagnostic contrast agent development have empowered researchers to noninvasively study lung function and pulmonary disorders. Investigators can identify, track, and quantify biological processes over time. In this review, we highlight the fundamental principles of bioluminescence, fluorescence, planar X-ray, X-ray computed tomography, magnetic resonance imaging, and nuclear imaging modalities (such as positron emission tomography and single photon emission computed tomography that have been successfully employed for the study of lung function and pulmonary disorders in a preclinical setting. The major principles, benefits, and applications of each imaging modality and technology are reviewed. Limitations and the future prospective of multimodal imaging in pulmonary physiology are also discussed. In vivo imaging bridges molecular biological studies, drug design and discovery, and the imaging field with modern medical practice, and, as such, will continue to be a mainstay in biomedical research.
KW - Epithelial sodium channels (ENaC) and fluid clearance
KW - Image molecular events in vivo
KW - Noninvasive lung imaging
KW - PAO-P1-lux bioluminescence
KW - PET/SPECT/CT
UR - http://www.scopus.com/inward/record.url?scp=84900823117&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84900823117&partnerID=8YFLogxK
U2 - 10.1152/ajplung.00007.2014
DO - 10.1152/ajplung.00007.2014
M3 - Review article
C2 - 24658139
AN - SCOPUS:84900823117
SN - 1040-0605
VL - 306
SP - L897-L914
JO - American Journal of Physiology - Lung Cellular and Molecular Physiology
JF - American Journal of Physiology - Lung Cellular and Molecular Physiology
IS - 10
ER -