TY - JOUR
T1 - Preliminary x-ray analysis of single crystals of ovalbumin and plakalbumin.
AU - Miller, M.
AU - Weinstein, J. N.
AU - Wlodawer, A.
N1 - Copyright:
Medline is the source for the citation and abstract of this record.
PY - 1983/5/10
Y1 - 1983/5/10
N2 - Two crystal forms of hen ovalbumin have been grown and were characterized by x-ray diffraction. Crystals of ovalbumin grown at pH 5.4 are monoclinic, belonging to the space group C2 (a = 116.5 A, b = 41.8 A, c = 70.5 A, beta = 101.5 degrees). At pH 5.8 or higher ovalbumin forms triclinic crystals of space group P1 (a = 62.0 A, b = 84.3 A, c = 72.2 A, alpha = 87.4 degrees, beta = 103.9 degrees, gamma = 108.7 degrees). These crystals are usually small and difficult to grow reproducibly. Crystals of plakalbumin (a proteolytic modification of ovalbumin) are much easier to grow and appear suitable for three-dimensional structure determination. Their space group is C2 (a = 99.9 A, b = 102.0 A, c = 79.3 A, beta = 112.1 degrees) with two plakalbumin molecules in each asymmetric unit.
AB - Two crystal forms of hen ovalbumin have been grown and were characterized by x-ray diffraction. Crystals of ovalbumin grown at pH 5.4 are monoclinic, belonging to the space group C2 (a = 116.5 A, b = 41.8 A, c = 70.5 A, beta = 101.5 degrees). At pH 5.8 or higher ovalbumin forms triclinic crystals of space group P1 (a = 62.0 A, b = 84.3 A, c = 72.2 A, alpha = 87.4 degrees, beta = 103.9 degrees, gamma = 108.7 degrees). These crystals are usually small and difficult to grow reproducibly. Crystals of plakalbumin (a proteolytic modification of ovalbumin) are much easier to grow and appear suitable for three-dimensional structure determination. Their space group is C2 (a = 99.9 A, b = 102.0 A, c = 79.3 A, beta = 112.1 degrees) with two plakalbumin molecules in each asymmetric unit.
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M3 - Article
C2 - 6853552
AN - SCOPUS:0021099465
SN - 0021-9258
VL - 258
SP - 5864
EP - 5866
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 9
ER -