Premature chromosome condensation. Conformational changes of chromatin associated with phytohemagglutinin stimulation of peripheral lymphocytes

W. N. Hittelman; P. N. Rao

doi:10.1016/0014-4827(76)90140-3

Premature chromosome condensation. Conformational changes of chromatin associated with phytohemagglutinin stimulation of peripheral lymphocytes

W. N. Hittelman, P. N. Rao

Experimental Therapeutics

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29 Scopus citations

Abstract

Phytohemagglutinin (PHA)-stimulated human peripheral blood lymphocytes were shown to be distinguishable from unstimulated lymphocytes by the technique of premature chromosome condensation (PCC). Greater than 70% of the PCC from lymphocytes stimulated by incubating with PHA for 18-22 h showed greatly extended PCC as compared with only 30% in the unstimulated cultures. This decondensation pattern of the PCC paralleled with the previously reported increase in the template activity of chromatin. The PCC method can be useful in determining the proliferative potential of bone marrows of leukemic patients during and after chemotherapy.

Original language	English (US)
Pages (from-to)	219-222
Number of pages	4
Journal	Experimental Cell Research
Volume	100
Issue number	2
DOIs	https://doi.org/10.1016/0014-4827(76)90140-3
State	Published - Jul 1976

ASJC Scopus subject areas

Cell Biology

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10.1016/0014-4827(76)90140-3

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title = "Premature chromosome condensation. Conformational changes of chromatin associated with phytohemagglutinin stimulation of peripheral lymphocytes",

abstract = "Phytohemagglutinin (PHA)-stimulated human peripheral blood lymphocytes were shown to be distinguishable from unstimulated lymphocytes by the technique of premature chromosome condensation (PCC). Greater than 70% of the PCC from lymphocytes stimulated by incubating with PHA for 18-22 h showed greatly extended PCC as compared with only 30% in the unstimulated cultures. This decondensation pattern of the PCC paralleled with the previously reported increase in the template activity of chromatin. The PCC method can be useful in determining the proliferative potential of bone marrows of leukemic patients during and after chemotherapy.",

author = "Hittelman, {W. N.} and Rao, {P. N.}",

note = "Funding Information: This research was supported in part by Grants CA-16480, CA-14528-02, CA-11520 from the NCI, NIH, USPHS and the Grant VC-163 from the ACS.",

year = "1976",

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doi = "10.1016/0014-4827(76)90140-3",

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T1 - Premature chromosome condensation. Conformational changes of chromatin associated with phytohemagglutinin stimulation of peripheral lymphocytes

AU - Hittelman, W. N.

AU - Rao, P. N.

N1 - Funding Information: This research was supported in part by Grants CA-16480, CA-14528-02, CA-11520 from the NCI, NIH, USPHS and the Grant VC-163 from the ACS.

PY - 1976/7

Y1 - 1976/7

N2 - Phytohemagglutinin (PHA)-stimulated human peripheral blood lymphocytes were shown to be distinguishable from unstimulated lymphocytes by the technique of premature chromosome condensation (PCC). Greater than 70% of the PCC from lymphocytes stimulated by incubating with PHA for 18-22 h showed greatly extended PCC as compared with only 30% in the unstimulated cultures. This decondensation pattern of the PCC paralleled with the previously reported increase in the template activity of chromatin. The PCC method can be useful in determining the proliferative potential of bone marrows of leukemic patients during and after chemotherapy.

AB - Phytohemagglutinin (PHA)-stimulated human peripheral blood lymphocytes were shown to be distinguishable from unstimulated lymphocytes by the technique of premature chromosome condensation (PCC). Greater than 70% of the PCC from lymphocytes stimulated by incubating with PHA for 18-22 h showed greatly extended PCC as compared with only 30% in the unstimulated cultures. This decondensation pattern of the PCC paralleled with the previously reported increase in the template activity of chromatin. The PCC method can be useful in determining the proliferative potential of bone marrows of leukemic patients during and after chemotherapy.

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Premature chromosome condensation. Conformational changes of chromatin associated with phytohemagglutinin stimulation of peripheral lymphocytes

Abstract

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