Preparation of mannan-protein conjugates using high-temperature glycation

Urszula Kanska; Renata Budzynska; Dmitry Nevozhay; Janusz Boratynski

doi:10.1042/BA20070024

Preparation of mannan-protein conjugates using high-temperature glycation

Urszula Kanska, Renata Budzynska, Dmitry Nevozhay, Janusz Boratynski

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

In the present paper, we describe a procedure for the preparation of mannan-protein conjugates. Mannan from Saccharomyces cerevisiae (baker's yeast) was oxidized by NaIO₄ and coupled with BSA by means of high-temperature glycation. Depending on the temperature and degree of mannan oxidation, the procedure enabled different levels of substitution to be obtained. For conjugate prepared under selected conditions, the average substitution level was 1.64 molecules of mannan per albumin molecule. The mannan-albumin conjugate was purified by means of ion-exchange chromatography on CM-cellulose at a pH below the pI of albumin. The influence of coupling conditions on protein activity was examined using urease as an example. Urease linked to mannan retained its activity. The mannan-protein conjugates can be used, for example, as carriers of therapeutic substances to macrophages.

Original language	English (US)
Pages (from-to)	57-64
Number of pages	8
Journal	Biotechnology and Applied Biochemistry
Volume	49
Issue number	1
DOIs	https://doi.org/10.1042/BA20070024
State	Published - Jan 2008
Externally published	Yes

Keywords

Albumin
Conjugate
Glycation
Mannan
Urease

ASJC Scopus subject areas

Biotechnology
Bioengineering
Molecular Medicine
Biomedical Engineering
Applied Microbiology and Biotechnology
Drug Discovery
Process Chemistry and Technology

Access to Document

10.1042/BA20070024

Cite this

@article{9cad28b2387e43769dcfa8d21cf03541,

title = "Preparation of mannan-protein conjugates using high-temperature glycation",

abstract = "In the present paper, we describe a procedure for the preparation of mannan-protein conjugates. Mannan from Saccharomyces cerevisiae (baker's yeast) was oxidized by NaIO4 and coupled with BSA by means of high-temperature glycation. Depending on the temperature and degree of mannan oxidation, the procedure enabled different levels of substitution to be obtained. For conjugate prepared under selected conditions, the average substitution level was 1.64 molecules of mannan per albumin molecule. The mannan-albumin conjugate was purified by means of ion-exchange chromatography on CM-cellulose at a pH below the pI of albumin. The influence of coupling conditions on protein activity was examined using urease as an example. Urease linked to mannan retained its activity. The mannan-protein conjugates can be used, for example, as carriers of therapeutic substances to macrophages.",

keywords = "Albumin, Conjugate, Glycation, Mannan, Urease",

author = "Urszula Kanska and Renata Budzynska and Dmitry Nevozhay and Janusz Boratynski",

year = "2008",

month = jan,

doi = "10.1042/BA20070024",

language = "English (US)",

volume = "49",

pages = "57--64",

journal = "Biotechnology and Applied Biochemistry",

issn = "0885-4513",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - Preparation of mannan-protein conjugates using high-temperature glycation

AU - Kanska, Urszula

AU - Budzynska, Renata

AU - Nevozhay, Dmitry

AU - Boratynski, Janusz

PY - 2008/1

Y1 - 2008/1

N2 - In the present paper, we describe a procedure for the preparation of mannan-protein conjugates. Mannan from Saccharomyces cerevisiae (baker's yeast) was oxidized by NaIO4 and coupled with BSA by means of high-temperature glycation. Depending on the temperature and degree of mannan oxidation, the procedure enabled different levels of substitution to be obtained. For conjugate prepared under selected conditions, the average substitution level was 1.64 molecules of mannan per albumin molecule. The mannan-albumin conjugate was purified by means of ion-exchange chromatography on CM-cellulose at a pH below the pI of albumin. The influence of coupling conditions on protein activity was examined using urease as an example. Urease linked to mannan retained its activity. The mannan-protein conjugates can be used, for example, as carriers of therapeutic substances to macrophages.

AB - In the present paper, we describe a procedure for the preparation of mannan-protein conjugates. Mannan from Saccharomyces cerevisiae (baker's yeast) was oxidized by NaIO4 and coupled with BSA by means of high-temperature glycation. Depending on the temperature and degree of mannan oxidation, the procedure enabled different levels of substitution to be obtained. For conjugate prepared under selected conditions, the average substitution level was 1.64 molecules of mannan per albumin molecule. The mannan-albumin conjugate was purified by means of ion-exchange chromatography on CM-cellulose at a pH below the pI of albumin. The influence of coupling conditions on protein activity was examined using urease as an example. Urease linked to mannan retained its activity. The mannan-protein conjugates can be used, for example, as carriers of therapeutic substances to macrophages.

KW - Albumin

KW - Conjugate

KW - Glycation

KW - Mannan

KW - Urease

UR - http://www.scopus.com/inward/record.url?scp=38349026449&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38349026449&partnerID=8YFLogxK

U2 - 10.1042/BA20070024

DO - 10.1042/BA20070024

M3 - Article

C2 - 17630955

AN - SCOPUS:38349026449

SN - 0885-4513

VL - 49

SP - 57

EP - 64

JO - Biotechnology and Applied Biochemistry

JF - Biotechnology and Applied Biochemistry

IS - 1

ER -

Preparation of mannan-protein conjugates using high-temperature glycation

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this