TY - JOUR
T1 - Preparing undergraduates to participate in the post-genome era
T2 - A capstone laboratory experience in proteomics
AU - Eberhardt, Eric S.
AU - Hansen, Johanna
AU - Riservato, Luke
AU - Cole, Melissa
AU - Smaglo, Brandon
AU - Szaniawski, Paul
PY - 2003/11
Y1 - 2003/11
N2 - Proteomics is one of the important new disciplines to emerge from the genome sequencing projects of the last decade. In order to introduce our students to the techniques and promise of this emerging field, a capstone laboratory experience has been developed. The exercise involves multiple aspects of proteomics research including microbial culturing methods, two-dimensional gel electrophoresis techniques, matrix-assisted laser desorption-ionization time-of-flight mass spectrometry, and database mining. Over a 12-week semester, students design their own experiments and apply a proteomic approach to investigate the heat shock response in Escherichia coli. In the trial presented in this article, students successfully identified several major heat shock proteins. The laboratory outlined here can be readily adapted to explore a wide variety of responses in metabolic pathways or responses resulting from other environmental insults or stresses. Additionally, the laboratory can be modified to explore the proteomes of organelles, tissues, and other model organisms.
AB - Proteomics is one of the important new disciplines to emerge from the genome sequencing projects of the last decade. In order to introduce our students to the techniques and promise of this emerging field, a capstone laboratory experience has been developed. The exercise involves multiple aspects of proteomics research including microbial culturing methods, two-dimensional gel electrophoresis techniques, matrix-assisted laser desorption-ionization time-of-flight mass spectrometry, and database mining. Over a 12-week semester, students design their own experiments and apply a proteomic approach to investigate the heat shock response in Escherichia coli. In the trial presented in this article, students successfully identified several major heat shock proteins. The laboratory outlined here can be readily adapted to explore a wide variety of responses in metabolic pathways or responses resulting from other environmental insults or stresses. Additionally, the laboratory can be modified to explore the proteomes of organelles, tissues, and other model organisms.
KW - Biochemistry laboratory
KW - Heat shock
KW - Matrix-assisted laser desorption-ionization time-of-flight mass spectrometry
KW - Molecular chaperones
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=0347504895&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0347504895&partnerID=8YFLogxK
U2 - 10.1002/bmb.2003.494031060291
DO - 10.1002/bmb.2003.494031060291
M3 - Review article
AN - SCOPUS:0347504895
SN - 1470-8175
VL - 31
SP - 402
EP - 409
JO - Biochemistry and Molecular Biology Education
JF - Biochemistry and Molecular Biology Education
IS - 6
ER -