Prevention of apoptosis by Bcl-2: Release of cytochrome c from mitochondria blocked

Jie Yang; Xuesong Liu; Kapil Bhalla; Caryn Naekyung Kim; Ana Maria Ibrado; Jiyang Cai; Tsung I. Peng; Dean P. Jones; Xiaodong Wang

doi:10.1126/science.275.5303.1129

Prevention of apoptosis by Bcl-2: Release of cytochrome c from mitochondria blocked

Jie Yang, Xuesong Liu, Kapil Bhalla, Caryn Naekyung Kim, Ana Maria Ibrado, Jiyang Cai, Tsung I. Peng, Dean P. Jones, Xiaodong Wang

Research output: Contribution to journal › Article › peer-review

4481 Scopus citations

Abstract

Bcl-2 is an integral membrane protein located mainly on the outer membrane of mitochondria. Overexpression of Bcl-2 prevents cells from undergoing apoptosis in response to a variety of stimuli. Cytosolic cytochrome c is necessary for the initiation of the apoptotic program, suggesting a possible connection between Bcl-2 and cytochrome c, which is normally located in the mitochondrial intermembrane space. Cells undergoing apoptosis were found to have an elevation of cytochrome c in the cytosol and a corresponding decrease in the mitochondria. Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria.

Original language	English (US)
Pages (from-to)	1129-1132
Number of pages	4
Journal	Science
Volume	275
Issue number	5303
DOIs	https://doi.org/10.1126/science.275.5303.1129
State	Published - Feb 21 1997
Externally published	Yes

ASJC Scopus subject areas

General

Access to Document

10.1126/science.275.5303.1129

Cite this

@article{49689de67bf940bba57c5b5b978ceed7,

title = "Prevention of apoptosis by Bcl-2: Release of cytochrome c from mitochondria blocked",

abstract = "Bcl-2 is an integral membrane protein located mainly on the outer membrane of mitochondria. Overexpression of Bcl-2 prevents cells from undergoing apoptosis in response to a variety of stimuli. Cytosolic cytochrome c is necessary for the initiation of the apoptotic program, suggesting a possible connection between Bcl-2 and cytochrome c, which is normally located in the mitochondrial intermembrane space. Cells undergoing apoptosis were found to have an elevation of cytochrome c in the cytosol and a corresponding decrease in the mitochondria. Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria.",

author = "Jie Yang and Xuesong Liu and Kapil Bhalla and Kim, {Caryn Naekyung} and Ibrado, {Ana Maria} and Jiyang Cai and Peng, {Tsung I.} and Jones, {Dean P.} and Xiaodong Wang",

year = "1997",

month = feb,

day = "21",

doi = "10.1126/science.275.5303.1129",

language = "English (US)",

volume = "275",

pages = "1129--1132",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "5303",

}

TY - JOUR

T1 - Prevention of apoptosis by Bcl-2

T2 - Release of cytochrome c from mitochondria blocked

AU - Yang, Jie

AU - Liu, Xuesong

AU - Bhalla, Kapil

AU - Kim, Caryn Naekyung

AU - Ibrado, Ana Maria

AU - Cai, Jiyang

AU - Peng, Tsung I.

AU - Jones, Dean P.

AU - Wang, Xiaodong

PY - 1997/2/21

Y1 - 1997/2/21

N2 - Bcl-2 is an integral membrane protein located mainly on the outer membrane of mitochondria. Overexpression of Bcl-2 prevents cells from undergoing apoptosis in response to a variety of stimuli. Cytosolic cytochrome c is necessary for the initiation of the apoptotic program, suggesting a possible connection between Bcl-2 and cytochrome c, which is normally located in the mitochondrial intermembrane space. Cells undergoing apoptosis were found to have an elevation of cytochrome c in the cytosol and a corresponding decrease in the mitochondria. Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria.

AB - Bcl-2 is an integral membrane protein located mainly on the outer membrane of mitochondria. Overexpression of Bcl-2 prevents cells from undergoing apoptosis in response to a variety of stimuli. Cytosolic cytochrome c is necessary for the initiation of the apoptotic program, suggesting a possible connection between Bcl-2 and cytochrome c, which is normally located in the mitochondrial intermembrane space. Cells undergoing apoptosis were found to have an elevation of cytochrome c in the cytosol and a corresponding decrease in the mitochondria. Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria.

UR - http://www.scopus.com/inward/record.url?scp=0031036872&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031036872&partnerID=8YFLogxK

U2 - 10.1126/science.275.5303.1129

DO - 10.1126/science.275.5303.1129

M3 - Article

C2 - 9027314

AN - SCOPUS:0031036872

SN - 0036-8075

VL - 275

SP - 1129

EP - 1132

JO - Science

JF - Science

IS - 5303

ER -

Prevention of apoptosis by Bcl-2: Release of cytochrome c from mitochondria blocked

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this