TY - JOUR
T1 - Preventive effects of lupeol on DMBA induced DNA alkylation damage in mouse skin
AU - Nigam, Nidhi
AU - Prasad, Sahdeo
AU - Shukla, Yogeshwer
N1 - Funding Information:
Authors express their gratitude towards Dr. C.M. Gupta, Director, Industrial Toxicology Research Centre, Lucknow, for his keen interest and support during the course of the study. Authors are also thankful to Department of Biotechnology, New Delhi for providing Junior Research Fellowship to Ms. Nidhi Nigam.
PY - 2007/11
Y1 - 2007/11
N2 - Mutations that occur through DNA strand breaks are the precursors of the variety of genetic disorders including cancer. Life style and dietary habits are considered as major determinants in causation and prevention of genetic diseases. Epidemiological and laboratory studies suggest that plant derived compounds have the potential to prevent a number of genetic diseases. Therefore, use of nutraceuticals can be an important and convenient tool for chemoprevention. Polyphenolic phytochemicals such as epigallocatechin gallate flavonoids quercetin, genistein, curcumin and resveratrol constitute a class of nutraceuticals with notable efficacy in preclinical models of carcinogenesis. Lupeol, a pentacyclic triterpene present in mango, is a biologically active compound that has been reported to possess a number of pharmacological properties in the in vivo and in vitro studies. In the present study, we investigated the effects of lupeol on 7,12-dimethylbenz[a]anthracene (DMBA), induced DNA strand breaks in mouse skin, using an alkaline unwinding assay. Increasing doses of lupeol (50-200 μg/mouse) were given topically, prior or after the single topical application of DMBA (100 μg/mouse) with the sampling time of 24, 48, 72 and 96 h, respectively. Both pre and post treatment of lupeol showed significant (p < 0.001) preventive effects in DMBA induced DNA strand breaks in dose and time dependent manner. The pre-treatment of lupeol at the dose of 200 μg/mouse showed 56.05% prevention, and post-treatment at the same dose showed 43.26% prevention, at 96 h time interval, against DMBA induced DNA strand breakage. The results suggest preventive effects of lupeol on DMBA induced DNA alkylation damage in Swiss albino mice.
AB - Mutations that occur through DNA strand breaks are the precursors of the variety of genetic disorders including cancer. Life style and dietary habits are considered as major determinants in causation and prevention of genetic diseases. Epidemiological and laboratory studies suggest that plant derived compounds have the potential to prevent a number of genetic diseases. Therefore, use of nutraceuticals can be an important and convenient tool for chemoprevention. Polyphenolic phytochemicals such as epigallocatechin gallate flavonoids quercetin, genistein, curcumin and resveratrol constitute a class of nutraceuticals with notable efficacy in preclinical models of carcinogenesis. Lupeol, a pentacyclic triterpene present in mango, is a biologically active compound that has been reported to possess a number of pharmacological properties in the in vivo and in vitro studies. In the present study, we investigated the effects of lupeol on 7,12-dimethylbenz[a]anthracene (DMBA), induced DNA strand breaks in mouse skin, using an alkaline unwinding assay. Increasing doses of lupeol (50-200 μg/mouse) were given topically, prior or after the single topical application of DMBA (100 μg/mouse) with the sampling time of 24, 48, 72 and 96 h, respectively. Both pre and post treatment of lupeol showed significant (p < 0.001) preventive effects in DMBA induced DNA strand breaks in dose and time dependent manner. The pre-treatment of lupeol at the dose of 200 μg/mouse showed 56.05% prevention, and post-treatment at the same dose showed 43.26% prevention, at 96 h time interval, against DMBA induced DNA strand breakage. The results suggest preventive effects of lupeol on DMBA induced DNA alkylation damage in Swiss albino mice.
KW - DMBA
KW - DNA alkaline unwinding assay
KW - DNA damage
KW - Lupeol
KW - Mouse skin
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U2 - 10.1016/j.fct.2007.06.002
DO - 10.1016/j.fct.2007.06.002
M3 - Article
C2 - 17637493
AN - SCOPUS:34548822395
SN - 0278-6915
VL - 45
SP - 2331
EP - 2335
JO - Food and Chemical Toxicology
JF - Food and Chemical Toxicology
IS - 11
ER -