TY - JOUR
T1 - Procollagen alpha 1 type I
T2 - A potential aide in histopathological grading of glioma
AU - Savaraj, Niramol
AU - Wu, Chunjing
AU - Landy, Howard
AU - Wangpaijit, Medhi
AU - Wei, Yingjie
AU - Kuo, M. Tien
AU - Robles, Carlos
AU - Furst, A. J.
AU - Lampidis, Theodore
AU - Feun, Lynn
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005
Y1 - 2005
N2 - Collagen type I production has been shown to play a role in malignant transformation. We examined procollagen type I expression in brain tumors and with histopathological grading. Expression levels of procollagen alpha 1 type 1 were determined in 5 glioma cell lines by RT-PCR, Northern, and Western blot analysis. In addition, 41 primary brain tumors and 2 metastatic lung cancers to the brain were examined by PCR. Of the 5 glioma cell line analyzed, 3 (glioma 1, SW-1783 and U-118) expressed procollagen alpha 1 type I and were sensitive to vitamin D3 (VD3). In contrast, 2 of the cell lines (U-373 and T-98G) lacked procollagen alpha 1 type 1 expression. In patients' samples, 14 of 15 anaplastic and low grade gliomas expressed procollagen alpha 1 type I, and 12 of the 14 expressed high levels. In contrast, only 12 of 21 high grade gliomas from patients expressed procollagen alpha 1 type1 and among these, only 4 of the 12 expressed high levels. Thus, there is an inversed correlation between procollagen alpha 1 type 1 expression and histopathological grading (R 2 = -0.56, p = 0.0005). Our data suggest that procollagen alpha 1 type I expression occurs more commonly in intermediate and low grade gliomas and may assist in histopathological grading.
AB - Collagen type I production has been shown to play a role in malignant transformation. We examined procollagen type I expression in brain tumors and with histopathological grading. Expression levels of procollagen alpha 1 type 1 were determined in 5 glioma cell lines by RT-PCR, Northern, and Western blot analysis. In addition, 41 primary brain tumors and 2 metastatic lung cancers to the brain were examined by PCR. Of the 5 glioma cell line analyzed, 3 (glioma 1, SW-1783 and U-118) expressed procollagen alpha 1 type I and were sensitive to vitamin D3 (VD3). In contrast, 2 of the cell lines (U-373 and T-98G) lacked procollagen alpha 1 type 1 expression. In patients' samples, 14 of 15 anaplastic and low grade gliomas expressed procollagen alpha 1 type I, and 12 of the 14 expressed high levels. In contrast, only 12 of 21 high grade gliomas from patients expressed procollagen alpha 1 type1 and among these, only 4 of the 12 expressed high levels. Thus, there is an inversed correlation between procollagen alpha 1 type 1 expression and histopathological grading (R 2 = -0.56, p = 0.0005). Our data suggest that procollagen alpha 1 type I expression occurs more commonly in intermediate and low grade gliomas and may assist in histopathological grading.
KW - Glioma
KW - Histopathological grading
KW - Procollagen
UR - http://www.scopus.com/inward/record.url?scp=33344466629&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33344466629&partnerID=8YFLogxK
U2 - 10.1080/07357900500276915
DO - 10.1080/07357900500276915
M3 - Article
C2 - 16305983
AN - SCOPUS:33344466629
SN - 0735-7907
VL - 23
SP - 577
EP - 581
JO - Cancer Investigation
JF - Cancer Investigation
IS - 7
ER -