Profiling Long Non-coding RNA expression Using Custom-Designed Microarray

Xinna Zhang, George A. Calin

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Long non-coding RNAs (lncRNAs) are an important class of pervasive genes involved in a variety of biological functions. The abnormal expression of lncRNAs has been implicated in a range of many human diseases, including cancer. To date, a small number of functional lncRNAs have been well characterized. lncRNA expression profiling may help to identify useful molecular biomarkers and targets for novel therapeutic approaches. In this chapter, we describe a highly efficient lncRNA expression profiling method using a custom-designed microarray.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages43-51
Number of pages9
DOIs
StatePublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2372
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Microarray profiling
  • cDNA labeling
  • cDNA synthesis
  • lncRNA expression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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