Protection of the hemopoietic system by Podophyllum hexandrum against gamma radiation-induced damage

Ravinder Kumar Sagar; Raman Chawla; Rajesh Arora; Shikha Singh; Bal Krishna; Rakesh Kumar Sharma; Satish Chandra Puri; Pankaj Singh; Raj Kumar; Ashok Kumar Sharma; Surender Singh; Jagdish Prasad; Vinay Gupta; Bilal Ahmed; Kanaya Lal Dhar; Haider Ali Khan; Manju Lata Gupta; Ghulam Nabi Qazi

doi:10.1055/s-2005-873148

Protection of the hemopoietic system by Podophyllum hexandrum against gamma radiation-induced damage

Ravinder Kumar Sagar, Raman Chawla, Rajesh Arora, Shikha Singh, Bal Krishna, Rakesh Kumar Sharma, Satish Chandra Puri, Pankaj Singh, Raj Kumar, Ashok Kumar Sharma, Surender Singh, Jagdish Prasad, Vinay Gupta, Bilal Ahmed, Kanaya Lal Dhar, Haider Ali Khan, Manju Lata Gupta, Ghulam Nabi Qazi

Experimental Radiation Oncology

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

A semi-purified extract of low-altitude Podophyllum hexandrum (REC-2001) containing a relatively low content of podophyllotoxin (3.25%) exhibited potent antioxidant ability in lipid media (at 1000 μg/mL against 0.25 kGy) and significant (p < 0.05) hydroxyl ion scavenging potential (78.83% at 500 μg/mL). In vitro investigations revealed the ability of REC-2001 to significantly (p < 0.05) reduce radiation-induced hemolysis (2 μg/mL; 46.184%) and nitric oxide scavenging levels (IC₅₀: 792 ± 1.25; μg/mL). Protection of the hemopoietic system of Strain 'A' mice administered 20 mg/kg BW REC-2001 30 min prior to lethal irradiation (10 Gy) was recorded and was mediated by free radical scavenging and lowering of lipid oxidation. Further studies investigating the effects of REC-2001 on stem cell modulation are warranted.

Original language	English (US)
Pages (from-to)	114-120
Number of pages	7
Journal	Planta Medica
Volume	72
Issue number	2
DOIs	https://doi.org/10.1055/s-2005-873148
State	Published - Feb 2006

Keywords

Antioxidant
Hemopoietic system
Lignan glycoside
Podophyllotoxin
Podophyllum hexandrum
Radioprotection

ASJC Scopus subject areas

Analytical Chemistry
Molecular Medicine
Pharmacology
Pharmaceutical Science
Drug Discovery
Complementary and alternative medicine
Organic Chemistry

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10.1055/s-2005-873148

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Sagar, R. K., Chawla, R., Arora, R., Singh, S., Krishna, B., Sharma, R. K., Puri, S. C., Singh, P., Kumar, R., Sharma, A. K., Singh, S., Prasad, J., Gupta, V., Ahmed, B., Dhar, K. L., Khan, H. A., Gupta, M. L., & Qazi, G. N. (2006). Protection of the hemopoietic system by Podophyllum hexandrum against gamma radiation-induced damage. Planta Medica, 72(2), 114-120. https://doi.org/10.1055/s-2005-873148

Sagar, RK, Chawla, R, Arora, R, Singh, S, Krishna, B, Sharma, RK, Puri, SC, Singh, P, Kumar, R, Sharma, AK, Singh, S, Prasad, J, Gupta, V, Ahmed, B, Dhar, KL, Khan, HA, Gupta, ML & Qazi, GN 2006, 'Protection of the hemopoietic system by Podophyllum hexandrum against gamma radiation-induced damage', Planta Medica, vol. 72, no. 2, pp. 114-120. https://doi.org/10.1055/s-2005-873148

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abstract = "A semi-purified extract of low-altitude Podophyllum hexandrum (REC-2001) containing a relatively low content of podophyllotoxin (3.25%) exhibited potent antioxidant ability in lipid media (at 1000 μg/mL against 0.25 kGy) and significant (p < 0.05) hydroxyl ion scavenging potential (78.83% at 500 μg/mL). In vitro investigations revealed the ability of REC-2001 to significantly (p < 0.05) reduce radiation-induced hemolysis (2 μg/mL; 46.184%) and nitric oxide scavenging levels (IC50: 792 ± 1.25; μg/mL). Protection of the hemopoietic system of Strain 'A' mice administered 20 mg/kg BW REC-2001 30 min prior to lethal irradiation (10 Gy) was recorded and was mediated by free radical scavenging and lowering of lipid oxidation. Further studies investigating the effects of REC-2001 on stem cell modulation are warranted.",

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AU - Sharma, Rakesh Kumar

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AU - Singh, Surender

AU - Prasad, Jagdish

AU - Gupta, Vinay

AU - Ahmed, Bilal

AU - Dhar, Kanaya Lal

AU - Khan, Haider Ali

AU - Gupta, Manju Lata

AU - Qazi, Ghulam Nabi

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Protection of the hemopoietic system by Podophyllum hexandrum against gamma radiation-induced damage

Abstract

Keywords

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