TY - JOUR
T1 - Purification and biochemical characterization of a protein-palmitoyl acyltransferase from human erythrocytes
AU - Das, Amit K.
AU - Dasgupta, Biplab
AU - Bhattacharya, Raja
AU - Basu, Joyoti
PY - 1997/4/25
Y1 - 1997/4/25
N2 - Protein palmitoylation involves the post-translational attachment of palmitate in thioester linkage to cysteine residues of proteins. The labile nature of the thioester linkage makes possible the palmitoylation- depalmitoylation cycles that have emerged in recent times as additions to the repertoire of cellular control mechanisms. However, detailed understanding of these cycles has been limited by the lack of knowledge of the transferases and thioesterases likely to be involved. Here, we describe the purification of a protein-palmitoyl acyltransferase (PAT) from human erythrocytes. PAT behaved as a peripheral membrane protein and catalyzed the attachment of palmitate in thioester linkage to the β-subunit of spectrin. On SDS- polyacrylamide gel electrophoresis, PAT appeared as a 70-kDa polypeptide. Antibody against this polypeptide could immunodeplete PAT activity from the crude extract, confirming the assignment of the 70-kDa polypeptide as PAT. PAT-mediated spectrin palmitoylation could be inhibited by nonradioactive palmitoyl-, myristoyl-, or stearoyl-CoA. The apparent K(m) for palmitoyl-CoA was 16 μM.
AB - Protein palmitoylation involves the post-translational attachment of palmitate in thioester linkage to cysteine residues of proteins. The labile nature of the thioester linkage makes possible the palmitoylation- depalmitoylation cycles that have emerged in recent times as additions to the repertoire of cellular control mechanisms. However, detailed understanding of these cycles has been limited by the lack of knowledge of the transferases and thioesterases likely to be involved. Here, we describe the purification of a protein-palmitoyl acyltransferase (PAT) from human erythrocytes. PAT behaved as a peripheral membrane protein and catalyzed the attachment of palmitate in thioester linkage to the β-subunit of spectrin. On SDS- polyacrylamide gel electrophoresis, PAT appeared as a 70-kDa polypeptide. Antibody against this polypeptide could immunodeplete PAT activity from the crude extract, confirming the assignment of the 70-kDa polypeptide as PAT. PAT-mediated spectrin palmitoylation could be inhibited by nonradioactive palmitoyl-, myristoyl-, or stearoyl-CoA. The apparent K(m) for palmitoyl-CoA was 16 μM.
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U2 - 10.1074/jbc.272.17.11021
DO - 10.1074/jbc.272.17.11021
M3 - Article
C2 - 9110994
AN - SCOPUS:0030890881
SN - 0021-9258
VL - 272
SP - 11021
EP - 11025
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 17
ER -