Purification of β-glucosidase activities from bovine spleen by affinity chromatography

Julian N. Kanfer; Richard A. Mumford; Srinivasa S. Raghavan; James Byrd

doi:10.1016/0003-2697(74)90145-6

Purification of β-glucosidase activities from bovine spleen by affinity chromatography

Julian N. Kanfer, Richard A. Mumford, Srinivasa S. Raghavan, James Byrd

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Bovine spleen β-d-glucosidase, glucosylceramide: β-d-glucosidase and glucosylsphingosine: β-d-glucosidase were purified by chromatography on a "gluconate" Sepharose column. Ten other lysosomal acid hydrolases, present in the preparation applied to the column, were absent from the glucosidase fraction.

Original language	English (US)
Pages (from-to)	200-205
Number of pages	6
Journal	Analytical Biochemistry
Volume	60
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(74)90145-6
State	Published - Jul 1974
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Purification of β-glucosidase activities from bovine spleen by affinity chromatography",

abstract = "Bovine spleen β-d-glucosidase, glucosylceramide: β-d-glucosidase and glucosylsphingosine: β-d-glucosidase were purified by chromatography on a {"}gluconate{"} Sepharose column. Ten other lysosomal acid hydrolases, present in the preparation applied to the column, were absent from the glucosidase fraction.",

author = "Kanfer, {Julian N.} and Mumford, {Richard A.} and Raghavan, {Srinivasa S.} and James Byrd",

note = "Funding Information: ACKNOWLEDGMENTS Supported by Grants from the USPHS HD 05515 and NS Child Health Project 906.",

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AU - Kanfer, Julian N.

AU - Mumford, Richard A.

AU - Raghavan, Srinivasa S.

AU - Byrd, James

N1 - Funding Information: ACKNOWLEDGMENTS Supported by Grants from the USPHS HD 05515 and NS Child Health Project 906.

PY - 1974/7

Y1 - 1974/7

N2 - Bovine spleen β-d-glucosidase, glucosylceramide: β-d-glucosidase and glucosylsphingosine: β-d-glucosidase were purified by chromatography on a "gluconate" Sepharose column. Ten other lysosomal acid hydrolases, present in the preparation applied to the column, were absent from the glucosidase fraction.

AB - Bovine spleen β-d-glucosidase, glucosylceramide: β-d-glucosidase and glucosylsphingosine: β-d-glucosidase were purified by chromatography on a "gluconate" Sepharose column. Ten other lysosomal acid hydrolases, present in the preparation applied to the column, were absent from the glucosidase fraction.

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JF - Analytical Biochemistry

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Purification of β-glucosidase activities from bovine spleen by affinity chromatography

Abstract

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