pZ402, an improved SV40-based shuttle vector containing a T-antigen mutant unable to interact with wild-type p53

Eliyahu Kraus; Louise C. Strong; Michael A. Tainsky

doi:10.1016/S0378-1119(98)00121-8

pZ402, an improved SV40-based shuttle vector containing a T-antigen mutant unable to interact with wild-type p53

Eliyahu Kraus, Louise C. Strong, Michael A. Tainsky

Genetics

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Shuttle vectors are useful tools for studying DNA replication and mutagenesis. SV40-based shuttle vectors are popular because of their ease of use and quick results. However, one complication with the use of SV40-based shuttle vectors is the interaction of cellular p53 protein with the T-antigen of SV40. Wild-type, but not mutant p53 has been shown to be involved in DNA replication and DNA repair. To address this concern, we have modified an SV40-based shuttle vector, pZ189, by exchanging the wt T-antigen for a mutant SV40 T-antigen, which is unable to bind with p53. This shuttle vector, pZ402, provides us with a tool to study DNA replication and genomic instability in cells with varying genetic backgrounds without interference from the interaction of T-antigen with p53.

Original language	English (US)
Pages (from-to)	229-234
Number of pages	6
Journal	Gene
Volume	211
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1119(98)00121-8
State	Published - May 12 1998

Keywords

DNA replication
Genomic instability

ASJC Scopus subject areas

Genetics

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10.1016/S0378-1119(98)00121-8

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title = "pZ402, an improved SV40-based shuttle vector containing a T-antigen mutant unable to interact with wild-type p53",

abstract = "Shuttle vectors are useful tools for studying DNA replication and mutagenesis. SV40-based shuttle vectors are popular because of their ease of use and quick results. However, one complication with the use of SV40-based shuttle vectors is the interaction of cellular p53 protein with the T-antigen of SV40. Wild-type, but not mutant p53 has been shown to be involved in DNA replication and DNA repair. To address this concern, we have modified an SV40-based shuttle vector, pZ189, by exchanging the wt T-antigen for a mutant SV40 T-antigen, which is unable to bind with p53. This shuttle vector, pZ402, provides us with a tool to study DNA replication and genomic instability in cells with varying genetic backgrounds without interference from the interaction of T-antigen with p53.",

keywords = "DNA replication, Genomic instability",

author = "Eliyahu Kraus and Strong, {Louise C.} and Tainsky, {Michael A.}",

note = "Funding Information: The authors wish to thank Dr A. Levine, Dr C. Findley (Princeton University) and Dr B. Vogelstien (Johns Hopkins University) for expression vectors, Dr K. Dixon for the pZ189 shuttle vector and the MBM7070 cell line (University of Cincinnati, OH), and Dr D. Simmons for the mutant SV40 large T vector. This work was supported in part by the National Institutes of Health Training Grant CA-09299 (EK) and the National Institutes of Health core grant CA-16672 to the MDACC and a grant from the National Institutes of Health CA-P01 34936 (MAT and LCS).",

year = "1998",

month = may,

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doi = "10.1016/S0378-1119(98)00121-8",

language = "English (US)",

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pages = "229--234",

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AU - Kraus, Eliyahu

AU - Strong, Louise C.

AU - Tainsky, Michael A.

N1 - Funding Information: The authors wish to thank Dr A. Levine, Dr C. Findley (Princeton University) and Dr B. Vogelstien (Johns Hopkins University) for expression vectors, Dr K. Dixon for the pZ189 shuttle vector and the MBM7070 cell line (University of Cincinnati, OH), and Dr D. Simmons for the mutant SV40 large T vector. This work was supported in part by the National Institutes of Health Training Grant CA-09299 (EK) and the National Institutes of Health core grant CA-16672 to the MDACC and a grant from the National Institutes of Health CA-P01 34936 (MAT and LCS).

PY - 1998/5/12

Y1 - 1998/5/12

N2 - Shuttle vectors are useful tools for studying DNA replication and mutagenesis. SV40-based shuttle vectors are popular because of their ease of use and quick results. However, one complication with the use of SV40-based shuttle vectors is the interaction of cellular p53 protein with the T-antigen of SV40. Wild-type, but not mutant p53 has been shown to be involved in DNA replication and DNA repair. To address this concern, we have modified an SV40-based shuttle vector, pZ189, by exchanging the wt T-antigen for a mutant SV40 T-antigen, which is unable to bind with p53. This shuttle vector, pZ402, provides us with a tool to study DNA replication and genomic instability in cells with varying genetic backgrounds without interference from the interaction of T-antigen with p53.

AB - Shuttle vectors are useful tools for studying DNA replication and mutagenesis. SV40-based shuttle vectors are popular because of their ease of use and quick results. However, one complication with the use of SV40-based shuttle vectors is the interaction of cellular p53 protein with the T-antigen of SV40. Wild-type, but not mutant p53 has been shown to be involved in DNA replication and DNA repair. To address this concern, we have modified an SV40-based shuttle vector, pZ189, by exchanging the wt T-antigen for a mutant SV40 T-antigen, which is unable to bind with p53. This shuttle vector, pZ402, provides us with a tool to study DNA replication and genomic instability in cells with varying genetic backgrounds without interference from the interaction of T-antigen with p53.

KW - DNA replication

KW - Genomic instability

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pZ402, an improved SV40-based shuttle vector containing a T-antigen mutant unable to interact with wild-type p53

Abstract

Keywords

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