TY - JOUR
T1 - Quality assurance of PASADENA hyperpolarization for 13C biomolecules
AU - Hövener, Jan Bernd
AU - Chekmenev, Eduard Y.
AU - Harris, Kent C.
AU - Perman, William H.
AU - Tran, Thao T.
AU - Ross, Brian D.
AU - Bhattacharya, Pratip
N1 - Funding Information:
Acknowledgments We thank the following for funding: NIH 1R21 CA118509 (PB), NCI 5R01CA122513 (BDR), NIH 1R01NS048589 (JBH, BDR), Rudi Schulte Research Institute (RSRI) (EYC, JBH), James G. Boswell Fellowship (PB, EYC), American Heart Association (PB), American Brain Tumor Association (PB), Tobacco Related Disease Research Program (PB), NARSAD (KH), Cancer Research and Prevention Foundation (EYC). We thank Dr. Daniel P. Weitekamp and Valerie A. Norton for assistance with the polarizer. We also thank Dr. William Opel for support of PASADENA program at HMRI, Dr. Scott Ross for providing convenient access to 14 T Varian high resolution solution NMR facility of Caltech. JBH thanks Drs. Peter Bachert and Wolfhard Semmler (German Cancer Research Center, DKFZ, Heidelberg, Germany) for PhD supervision. PB and BDR thank Drs. Oskar Axelsson, Haukur Johannesson, Magnus Karlsson for advice and training with the parahydrogen polarizer, in Malmo and provided for this work under loan agreement between HMRI and GE Healthcare, established by Dr. Klaes Golman.
PY - 2009/4
Y1 - 2009/4
N2 - Object: Define MR quality assurance procedures for maximal PASADENA hyperpolarization of a biological 13C molecular imaging reagent. Materials and methods: An automated PASADENA polarizer and a parahydrogen generator were installed. 13C enriched hydroxyethyl acrylate, 1- 13C, 2,3,3-d3 (HEA), was converted to hyperpolarized hydroxyethyl propionate, 1-13C, 2,3,3-d3 (HEP) and fumaric acid, 1-13C, 2,3-d2 (FUM) to hyperpolarized succinic acid, 1-13C, 2,3-d2 (SUC), by reaction with parahydrogen and norbornadiene rhodium catalyst. Incremental optimization of successive steps in PASADENA was implemented. MR spectra and in vivo images of hyperpolarized 13C imaging agents were acquired at 1.5 and 4.7 T. Results: Application of quality assurance (QA) criteria resulted in incremental optimization of the individual steps in PASADENA implementation. Optimal hyperpolarization of HEP of P = 20% was achieved by calibration of the NMR unit of the polarizer (B 0 field strength ± 0.002 mT). Mean hyperpolarization of SUC, P = [15.3 ± 1.9]% (N = 16) in D 2O, and P = [12.8 ± 3.1]% (N = 12) in H 2O, was achieved every 5-8 min (range 13-20%). An in vivo 13C succinate image of a rat was produced. Conclusion: PASADENA spin hyperpolarization of SUC to 15.3% in average was demonstrated (37,400 fold signal enhancement at 4.7 T). The biological fate of 13C succinate, a normally occurring cellular intermediate, might be monitored with enhanced sensitivity.
AB - Object: Define MR quality assurance procedures for maximal PASADENA hyperpolarization of a biological 13C molecular imaging reagent. Materials and methods: An automated PASADENA polarizer and a parahydrogen generator were installed. 13C enriched hydroxyethyl acrylate, 1- 13C, 2,3,3-d3 (HEA), was converted to hyperpolarized hydroxyethyl propionate, 1-13C, 2,3,3-d3 (HEP) and fumaric acid, 1-13C, 2,3-d2 (FUM) to hyperpolarized succinic acid, 1-13C, 2,3-d2 (SUC), by reaction with parahydrogen and norbornadiene rhodium catalyst. Incremental optimization of successive steps in PASADENA was implemented. MR spectra and in vivo images of hyperpolarized 13C imaging agents were acquired at 1.5 and 4.7 T. Results: Application of quality assurance (QA) criteria resulted in incremental optimization of the individual steps in PASADENA implementation. Optimal hyperpolarization of HEP of P = 20% was achieved by calibration of the NMR unit of the polarizer (B 0 field strength ± 0.002 mT). Mean hyperpolarization of SUC, P = [15.3 ± 1.9]% (N = 16) in D 2O, and P = [12.8 ± 3.1]% (N = 12) in H 2O, was achieved every 5-8 min (range 13-20%). An in vivo 13C succinate image of a rat was produced. Conclusion: PASADENA spin hyperpolarization of SUC to 15.3% in average was demonstrated (37,400 fold signal enhancement at 4.7 T). The biological fate of 13C succinate, a normally occurring cellular intermediate, might be monitored with enhanced sensitivity.
KW - C MRI
KW - Fumarate
KW - Hyperpolarization
KW - PASADENA
KW - Parahydrogen
KW - Succinate
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U2 - 10.1007/s10334-008-0154-y
DO - 10.1007/s10334-008-0154-y
M3 - Article
C2 - 19067009
AN - SCOPUS:63949085031
SN - 0968-5243
VL - 22
SP - 123
EP - 134
JO - Magnetic Resonance Materials in Physics, Biology and Medicine
JF - Magnetic Resonance Materials in Physics, Biology and Medicine
IS - 2
ER -