Abstract
Fungal specific CD154+ T-cells have been described as a biomarker in invasive aspergillosis. The influence of sample storage on the detection of these cells was assessed. Six-hour delay prior to PBMC isolation is associated with an 18% decrease of cell viability and alterations of the cellular composition of the sample. This results in 87% reduction of CD154+ A. fumigatus specific cells due to reduced assay sensitivity and increased background values in unstimulated samples. If prompt cell measurement is not feasible, isolated PBMCs can be frozen (at -20°C and -80°C) and processed later with comparable assay reliability (mean value fresh vs. thawing: 0.126, 0.133; Pearson-Coefficient: 0.962).
Original language | English (US) |
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Pages (from-to) | 223-227 |
Number of pages | 5 |
Journal | Medical mycology |
Volume | 55 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1 2017 |
Externally published | Yes |
Keywords
- Aspergillus fumigatus
- Biomarker
- Immune response
- Immunology
ASJC Scopus subject areas
- Infectious Diseases