TY - JOUR
T1 - Quantitation of steroid hormones in thin fresh frozen tissue sections
AU - Blonder, Josip
AU - Johann, Donald J.
AU - Veenstra, Timothy D.
AU - Xiao, Zhen
AU - Emmert-Buck, Michael R.
AU - Ziegler, Regina G.
AU - Rodriguez-Canales, Jaime
AU - Hanson, Jeffrey A.
AU - Xu, Xia
PY - 2008/11/15
Y1 - 2008/11/15
N2 - As analytical technologies in proteomics and metabolomics continue to mature, there is an increasing need to apply these to clinically relevant biologic samples. In this study, a liquid chromatography-tandem mass spectrometry method that utilizes selected reaction monitoring was used to measure the absolute quantity of estrogens and estrogen metabolites and testosterone in 8-μm tissue sections obtained from a fresh frozen lymph node tumor infiltrated by metastatic breast carcinoma. Total (conjugated plus unconjugated) and unconjugated levels of these steroid hormones were measured using two cohorts, each containing five adjacent serial sections cut from this tumor. The results were highly reproducible across replicate samples, showing that typical histological tissue sections represent an important sample type for the measurement of these specific metabolites.
AB - As analytical technologies in proteomics and metabolomics continue to mature, there is an increasing need to apply these to clinically relevant biologic samples. In this study, a liquid chromatography-tandem mass spectrometry method that utilizes selected reaction monitoring was used to measure the absolute quantity of estrogens and estrogen metabolites and testosterone in 8-μm tissue sections obtained from a fresh frozen lymph node tumor infiltrated by metastatic breast carcinoma. Total (conjugated plus unconjugated) and unconjugated levels of these steroid hormones were measured using two cohorts, each containing five adjacent serial sections cut from this tumor. The results were highly reproducible across replicate samples, showing that typical histological tissue sections represent an important sample type for the measurement of these specific metabolites.
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U2 - 10.1021/ac801402a
DO - 10.1021/ac801402a
M3 - Article
C2 - 18937426
AN - SCOPUS:56449114313
SN - 0003-2700
VL - 80
SP - 8845
EP - 8852
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 22
ER -