Quantitative Electrochemical DNA Microarray on a Monolith Electrode with Ten Attomolar Sensitivity, 100 % Specificity, and Zero Background

Shobana Raghunath; Abhijeet Prasad; Rahul Tevatia; Jillian R. Gunther; Santanu Roy; Sunil Krishnan; Ravi F. Saraf

doi:10.1002/celc.201700983

Quantitative Electrochemical DNA Microarray on a Monolith Electrode with Ten Attomolar Sensitivity, 100 % Specificity, and Zero Background

Shobana Raghunath, Abhijeet Prasad, Rahul Tevatia, Jillian R. Gunther, Santanu Roy, Sunil Krishnan, Ravi F. Saraf

Radiation Oncology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Circulating microRNA are promising diagnostic and prognostic biomarkers of disease in quantitative blood tests. A label-free, PCR-free, electrochemical microarray technology on a monolith electrode is described, with 10 attomolar (aM) sensitivity and responsiveness to binding of <1 zeptomole of target to immobilized ssDNA probes with zero background. Specificity is 100 % in a mixture with five nonspecific miRNA each with a 10³-fold higher concentration. Direct measurement on plasma-derived miRNA without cDNA conversion and PCR demonstrated multiplexing and near-ideal quantitative correlation with an equivalent pure sample. The dynamic range is a target concentration ranging from 10⁻² to 10³ femtomolar (fM). This PCR-free novel technology can be applied as a test for cancer diagnosis/prognosis to detect 10³ copies of a miRNA sequence in RNA extracted from 100 μL of plasma.

Original language	English (US)
Pages (from-to)	429-433
Number of pages	5
Journal	ChemElectroChem
Volume	5
Issue number	3
DOIs	https://doi.org/10.1002/celc.201700983
State	Published - Feb 2018

Keywords

DNA sequencing
blood test
cancer diagnosis
electrochemical analysis
microRNA
microarray

ASJC Scopus subject areas

Catalysis
Electrochemistry

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10.1002/celc.201700983

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title = "Quantitative Electrochemical DNA Microarray on a Monolith Electrode with Ten Attomolar Sensitivity, 100 % Specificity, and Zero Background",

abstract = "Circulating microRNA are promising diagnostic and prognostic biomarkers of disease in quantitative blood tests. A label-free, PCR-free, electrochemical microarray technology on a monolith electrode is described, with 10 attomolar (aM) sensitivity and responsiveness to binding of <1 zeptomole of target to immobilized ssDNA probes with zero background. Specificity is 100 % in a mixture with five nonspecific miRNA each with a 103-fold higher concentration. Direct measurement on plasma-derived miRNA without cDNA conversion and PCR demonstrated multiplexing and near-ideal quantitative correlation with an equivalent pure sample. The dynamic range is a target concentration ranging from 10−2 to 103 femtomolar (fM). This PCR-free novel technology can be applied as a test for cancer diagnosis/prognosis to detect 103 copies of a miRNA sequence in RNA extracted from 100 μL of plasma.",

keywords = "DNA sequencing, blood test, cancer diagnosis, electrochemical analysis, microRNA, microarray",

author = "Shobana Raghunath and Abhijeet Prasad and Rahul Tevatia and Gunther, {Jillian R.} and Santanu Roy and Sunil Krishnan and Saraf, {Ravi F.}",

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year = "2018",

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doi = "10.1002/celc.201700983",

language = "English (US)",

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AU - Raghunath, Shobana

AU - Prasad, Abhijeet

AU - Tevatia, Rahul

AU - Gunther, Jillian R.

AU - Roy, Santanu

AU - Krishnan, Sunil

AU - Saraf, Ravi F.

PY - 2018/2

Y1 - 2018/2

N2 - Circulating microRNA are promising diagnostic and prognostic biomarkers of disease in quantitative blood tests. A label-free, PCR-free, electrochemical microarray technology on a monolith electrode is described, with 10 attomolar (aM) sensitivity and responsiveness to binding of <1 zeptomole of target to immobilized ssDNA probes with zero background. Specificity is 100 % in a mixture with five nonspecific miRNA each with a 103-fold higher concentration. Direct measurement on plasma-derived miRNA without cDNA conversion and PCR demonstrated multiplexing and near-ideal quantitative correlation with an equivalent pure sample. The dynamic range is a target concentration ranging from 10−2 to 103 femtomolar (fM). This PCR-free novel technology can be applied as a test for cancer diagnosis/prognosis to detect 103 copies of a miRNA sequence in RNA extracted from 100 μL of plasma.

AB - Circulating microRNA are promising diagnostic and prognostic biomarkers of disease in quantitative blood tests. A label-free, PCR-free, electrochemical microarray technology on a monolith electrode is described, with 10 attomolar (aM) sensitivity and responsiveness to binding of <1 zeptomole of target to immobilized ssDNA probes with zero background. Specificity is 100 % in a mixture with five nonspecific miRNA each with a 103-fold higher concentration. Direct measurement on plasma-derived miRNA without cDNA conversion and PCR demonstrated multiplexing and near-ideal quantitative correlation with an equivalent pure sample. The dynamic range is a target concentration ranging from 10−2 to 103 femtomolar (fM). This PCR-free novel technology can be applied as a test for cancer diagnosis/prognosis to detect 103 copies of a miRNA sequence in RNA extracted from 100 μL of plasma.

KW - DNA sequencing

KW - blood test

KW - cancer diagnosis

KW - electrochemical analysis

KW - microRNA

KW - microarray

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Quantitative Electrochemical DNA Microarray on a Monolith Electrode with Ten Attomolar Sensitivity, 100 % Specificity, and Zero Background

Abstract

Keywords

ASJC Scopus subject areas

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