Quantitative real-time imaging of glutathione

Xiqian Jiang; Jianwei Chen; Aleksandar Bajić; Chengwei Zhang; Xianzhou Song; Shaina L. Carroll; Zhao Lin Cai; Meiling Tang; Mingshan Xue; Ninghui Cheng; Christian P. Schaaf; Feng Li; Kevin R. MacKenzie; Allan Chris M. Ferreon; Fan Xia; Meng C. Wang; Mirjana Maletić-Savatić; Jin Wang

doi:10.1038/ncomms16087

Quantitative real-time imaging of glutathione

Xiqian Jiang, Jianwei Chen, Aleksandar Bajić, Chengwei Zhang, Xianzhou Song, Shaina L. Carroll, Zhao Lin Cai, Meiling Tang, Mingshan Xue, Ninghui Cheng, Christian P. Schaaf, Feng Li, Kevin R. MacKenzie, Allan Chris M. Ferreon, Fan Xia, Meng C. Wang, Mirjana Maletić-Savatić, Jin Wang

Research output: Contribution to journal › Article › peer-review

190 Scopus citations

Abstract

Glutathione plays many important roles in biological processes; however, the dynamic changes of glutathione concentrations in living cells remain largely unknown. Here, we report a reversible reaction-based fluorescent probe-designated as RealThiol (RT)-that can quantitatively monitor the real-time glutathione dynamics in living cells. Using RT, we observe enhanced antioxidant capability of activated neurons and dynamic glutathione changes during ferroptosis. RT is thus a versatile tool that can be used for both confocal microscopy and flow cytometry based high-throughput quantification of glutathione levels in single cells. We envision that this new glutathione probe will enable opportunities to study glutathione dynamics and transportation and expand our understanding of the physiological and pathological roles of glutathione in living cells.

Original language	English (US)
Article number	16087
Journal	Nature communications
Volume	8
DOIs	https://doi.org/10.1038/ncomms16087
State	Published - Jul 13 2017
Externally published	Yes

ASJC Scopus subject areas

General Chemistry
General Biochemistry, Genetics and Molecular Biology
General Physics and Astronomy

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10.1038/ncomms16087

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title = "Quantitative real-time imaging of glutathione",

abstract = "Glutathione plays many important roles in biological processes; however, the dynamic changes of glutathione concentrations in living cells remain largely unknown. Here, we report a reversible reaction-based fluorescent probe-designated as RealThiol (RT)-that can quantitatively monitor the real-time glutathione dynamics in living cells. Using RT, we observe enhanced antioxidant capability of activated neurons and dynamic glutathione changes during ferroptosis. RT is thus a versatile tool that can be used for both confocal microscopy and flow cytometry based high-throughput quantification of glutathione levels in single cells. We envision that this new glutathione probe will enable opportunities to study glutathione dynamics and transportation and expand our understanding of the physiological and pathological roles of glutathione in living cells.",

author = "Xiqian Jiang and Jianwei Chen and Aleksandar Baji{\'c} and Chengwei Zhang and Xianzhou Song and Carroll, {Shaina L.} and Cai, {Zhao Lin} and Meiling Tang and Mingshan Xue and Ninghui Cheng and Schaaf, {Christian P.} and Feng Li and MacKenzie, {Kevin R.} and Ferreon, {Allan Chris M.} and Fan Xia and Wang, {Meng C.} and Mirjana Maleti{\'c}-Savati{\'c} and Jin Wang",

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doi = "10.1038/ncomms16087",

language = "English (US)",

volume = "8",

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T1 - Quantitative real-time imaging of glutathione

AU - Jiang, Xiqian

AU - Chen, Jianwei

AU - Bajić, Aleksandar

AU - Zhang, Chengwei

AU - Song, Xianzhou

AU - Carroll, Shaina L.

AU - Cai, Zhao Lin

AU - Tang, Meiling

AU - Xue, Mingshan

AU - Cheng, Ninghui

AU - Schaaf, Christian P.

AU - Li, Feng

AU - MacKenzie, Kevin R.

AU - Ferreon, Allan Chris M.

AU - Xia, Fan

AU - Wang, Meng C.

AU - Maletić-Savatić, Mirjana

AU - Wang, Jin

PY - 2017/7/13

Y1 - 2017/7/13

N2 - Glutathione plays many important roles in biological processes; however, the dynamic changes of glutathione concentrations in living cells remain largely unknown. Here, we report a reversible reaction-based fluorescent probe-designated as RealThiol (RT)-that can quantitatively monitor the real-time glutathione dynamics in living cells. Using RT, we observe enhanced antioxidant capability of activated neurons and dynamic glutathione changes during ferroptosis. RT is thus a versatile tool that can be used for both confocal microscopy and flow cytometry based high-throughput quantification of glutathione levels in single cells. We envision that this new glutathione probe will enable opportunities to study glutathione dynamics and transportation and expand our understanding of the physiological and pathological roles of glutathione in living cells.

AB - Glutathione plays many important roles in biological processes; however, the dynamic changes of glutathione concentrations in living cells remain largely unknown. Here, we report a reversible reaction-based fluorescent probe-designated as RealThiol (RT)-that can quantitatively monitor the real-time glutathione dynamics in living cells. Using RT, we observe enhanced antioxidant capability of activated neurons and dynamic glutathione changes during ferroptosis. RT is thus a versatile tool that can be used for both confocal microscopy and flow cytometry based high-throughput quantification of glutathione levels in single cells. We envision that this new glutathione probe will enable opportunities to study glutathione dynamics and transportation and expand our understanding of the physiological and pathological roles of glutathione in living cells.

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JO - Nature communications

JF - Nature communications

M1 - 16087

ER -

Quantitative real-time imaging of glutathione

Abstract

ASJC Scopus subject areas

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