@inbook{f4d9dce2b15844dfa2ae6ea92677a776,
title = "Quantitative real-time PCR: Recent advances",
abstract = "Quantitative real-time polymerase chain reaction is a technique for simultaneous amplification and product quantification of a target DNA as the process takes place in real time in a “closed-tube” system. Although this technique can provide an absolute quantification of the initial template copy number, quantification relative to a control sample or second sequence is typically adequate. The quantification process employs melting curve analysis and/or fluorescent detection systems and can provide amplification and genotyping in a relatively short time. Here we describe the properties and uses of various fluorescent detection systems used for quantifi cation.",
keywords = "Fluorescent genotyping, Hybridization probe, Intercalation probe, Minimal residual disease, Molecular beacon, Nucleic acid quantification, Polymerase chain reaction (PCR), Quantitative PCR, Real-time PCR, q-PCR",
author = "Charanjeet Singh and Sinchita Roy-Chowdhuri",
year = "2016",
month = feb,
day = "1",
doi = "10.1007/978-1-4939-3360-0_15",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "161--176",
booktitle = "Methods in Molecular Biology",
}