R26R-GR: A Cre-Activable Dual Fluorescent Protein Reporter Mouse

You Tzung Chen; Ming Shian Tsai; Tsung Lin Yang; Amy Tsu Ku; Ke Han Huang; Cheng Yen Huang; Fu Ju Chou; Hsiang Hsuan Fan; Jin Bon Hong; Shuo Ting Yen; Wei Le Wang; Chang Ching Lin; Yu Chen Hsu; Kang Yi Su; I. Chang Su; Chuan Wei Jang; Richard R. Behringer; Rebecca Favaro; Silvia K. Nicolis; Chung Liang Chien; Shu Wha Lin; I. Shing Yu

doi:10.1371/journal.pone.0046171

R26R-GR: A Cre-Activable Dual Fluorescent Protein Reporter Mouse

You Tzung Chen, Ming Shian Tsai, Tsung Lin Yang, Amy Tsu Ku, Ke Han Huang, Cheng Yen Huang, Fu Ju Chou, Hsiang Hsuan Fan, Jin Bon Hong, Shuo Ting Yen, Wei Le Wang, Chang Ching Lin, Yu Chen Hsu, Kang Yi Su, I. Chang Su, Chuan Wei Jang, Richard R. Behringer, Rebecca Favaro, Silvia K. Nicolis, Chung Liang ChienShu Wha Lin, I. Shing Yu

Genetics

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13 Scopus citations

Abstract

Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level. Here we generate a R26 dual fluorescent protein reporter mouse, activated by Cre-mediated DNA recombination, labeling target cells with a chromatin-specific enhanced green fluorescence protein (EGFP) and a plasma membrane-anchored monomeric cherry fluorescent protein (mCherry). This dual labeling allows the visualization of mitotic events, cell shapes and intracellular vesicle behaviors. We expect this reporter mouse to have a wide application in developmental biology studies, transplantation experiments as well as cancer/stem cell lineage tracing.

Original language	English (US)
Article number	e46171
Journal	PloS one
Volume	7
Issue number	9
DOIs	https://doi.org/10.1371/journal.pone.0046171
State	Published - Sep 25 2012

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Chen, Y. T., Tsai, M. S., Yang, T. L., Ku, A. T., Huang, K. H., Huang, C. Y., Chou, F. J., Fan, H. H., Hong, J. B., Yen, S. T., Wang, W. L., Lin, C. C., Hsu, Y. C., Su, K. Y., Su, I. C., Jang, C. W., Behringer, R. R., Favaro, R., Nicolis, S. K., ... Yu, I. S. (2012). R26R-GR: A Cre-Activable Dual Fluorescent Protein Reporter Mouse. PloS one, 7(9), Article e46171. https://doi.org/10.1371/journal.pone.0046171

Chen, YT, Tsai, MS, Yang, TL, Ku, AT, Huang, KH, Huang, CY, Chou, FJ, Fan, HH, Hong, JB, Yen, ST, Wang, WL, Lin, CC, Hsu, YC, Su, KY, Su, IC, Jang, CW, Behringer, RR, Favaro, R, Nicolis, SK, Chien, CL, Lin, SW & Yu, IS 2012, 'R26R-GR: A Cre-Activable Dual Fluorescent Protein Reporter Mouse', PloS one, vol. 7, no. 9, e46171. https://doi.org/10.1371/journal.pone.0046171

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abstract = "Green fluorescent protein (GFP) and its derivatives are the most widely used molecular reporters for live cell imagining. The development of organelle-specific fusion fluorescent proteins improves the labeling resolution to a higher level. Here we generate a R26 dual fluorescent protein reporter mouse, activated by Cre-mediated DNA recombination, labeling target cells with a chromatin-specific enhanced green fluorescence protein (EGFP) and a plasma membrane-anchored monomeric cherry fluorescent protein (mCherry). This dual labeling allows the visualization of mitotic events, cell shapes and intracellular vesicle behaviors. We expect this reporter mouse to have a wide application in developmental biology studies, transplantation experiments as well as cancer/stem cell lineage tracing.",

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AU - Huang, Cheng Yen

AU - Chou, Fu Ju

AU - Fan, Hsiang Hsuan

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AU - Yen, Shuo Ting

AU - Wang, Wei Le

AU - Lin, Chang Ching

AU - Hsu, Yu Chen

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AU - Jang, Chuan Wei

AU - Behringer, Richard R.

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R26R-GR: A Cre-Activable Dual Fluorescent Protein Reporter Mouse

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