Rad51C-deficient CL-V4B cells exhibit normal levels of mitomycin C-induced SCEs but reduced levels of UVC-induced SCEs

Andrzej Wojcik; Lubomir Stoilov; Irena Szumiel; Randy Legerski; Günter Obe

doi:10.1016/j.bbrc.2004.11.113

Rad51C-deficient CL-V4B cells exhibit normal levels of mitomycin C-induced SCEs but reduced levels of UVC-induced SCEs

Andrzej Wojcik, Lubomir Stoilov, Irena Szumiel, Randy Legerski, Günter Obe

Genetics

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The mechanisms of sister chromatid exchanges (SCEs) are not known. One hypothesis is that SCE is a manifestation of Rad51-dependent homologous recombination repair. In order to test this hypothesis, we have compared the frequencies of SCEs induced by mitomycin C (MMC) and 254 nm ultraviolet radiation (UVC) in wt V79B and the Rad51C-deficient CL-V4B cells. SCEs were analysed in the first (M1) and second (M2) post-treatment mitoses. In M1 MMC induced the same frequencies of SCEs in CL-V4B and V79B cells, while the UVC-induced SCE frequencies were lower in CL-V4B than V79B cells. In CL-V4B cells, MMC-induced SCEs were higher in M2 than in M1, suggesting that interstrand cross-links (ICL) are either not removed completely or are transformed into another form of DNA damage that persists until the next cell cycle. We suggest that SCEs may represent a mechanism to bypass MMC-induced ICL without their removal.

Original language	English (US)
Pages (from-to)	805-810
Number of pages	6
Journal	Biochemical and biophysical research communications
Volume	326
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2004.11.113
State	Published - Jan 28 2005

Keywords

Bromodeoxyuridine
Homologous recombination
Mitomycin C
Rad51C
Sister chromatid exchanges
UV radiation

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2004.11.113

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title = "Rad51C-deficient CL-V4B cells exhibit normal levels of mitomycin C-induced SCEs but reduced levels of UVC-induced SCEs",

abstract = "The mechanisms of sister chromatid exchanges (SCEs) are not known. One hypothesis is that SCE is a manifestation of Rad51-dependent homologous recombination repair. In order to test this hypothesis, we have compared the frequencies of SCEs induced by mitomycin C (MMC) and 254 nm ultraviolet radiation (UVC) in wt V79B and the Rad51C-deficient CL-V4B cells. SCEs were analysed in the first (M1) and second (M2) post-treatment mitoses. In M1 MMC induced the same frequencies of SCEs in CL-V4B and V79B cells, while the UVC-induced SCE frequencies were lower in CL-V4B than V79B cells. In CL-V4B cells, MMC-induced SCEs were higher in M2 than in M1, suggesting that interstrand cross-links (ICL) are either not removed completely or are transformed into another form of DNA damage that persists until the next cell cycle. We suggest that SCEs may represent a mechanism to bypass MMC-induced ICL without their removal.",

keywords = "Bromodeoxyuridine, Homologous recombination, Mitomycin C, Rad51C, Sister chromatid exchanges, UV radiation",

author = "Andrzej Wojcik and Lubomir Stoilov and Irena Szumiel and Randy Legerski and G{\"u}nter Obe",

note = "Funding Information: We are grateful to Prof. M.Z. Zdzienicka for providing the CL-V4B and V79B cells, and to Dr. R.H. Heflich for valuable editorial help. The work was partly supported by the statutory grant to the INCT (I.S.) from the Polish Ministry of Science and Technology and by an EMBO fellowship to L.S. ",

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T1 - Rad51C-deficient CL-V4B cells exhibit normal levels of mitomycin C-induced SCEs but reduced levels of UVC-induced SCEs

AU - Wojcik, Andrzej

AU - Stoilov, Lubomir

AU - Szumiel, Irena

AU - Legerski, Randy

AU - Obe, Günter

N1 - Funding Information: We are grateful to Prof. M.Z. Zdzienicka for providing the CL-V4B and V79B cells, and to Dr. R.H. Heflich for valuable editorial help. The work was partly supported by the statutory grant to the INCT (I.S.) from the Polish Ministry of Science and Technology and by an EMBO fellowship to L.S.

PY - 2005/1/28

Y1 - 2005/1/28

N2 - The mechanisms of sister chromatid exchanges (SCEs) are not known. One hypothesis is that SCE is a manifestation of Rad51-dependent homologous recombination repair. In order to test this hypothesis, we have compared the frequencies of SCEs induced by mitomycin C (MMC) and 254 nm ultraviolet radiation (UVC) in wt V79B and the Rad51C-deficient CL-V4B cells. SCEs were analysed in the first (M1) and second (M2) post-treatment mitoses. In M1 MMC induced the same frequencies of SCEs in CL-V4B and V79B cells, while the UVC-induced SCE frequencies were lower in CL-V4B than V79B cells. In CL-V4B cells, MMC-induced SCEs were higher in M2 than in M1, suggesting that interstrand cross-links (ICL) are either not removed completely or are transformed into another form of DNA damage that persists until the next cell cycle. We suggest that SCEs may represent a mechanism to bypass MMC-induced ICL without their removal.

AB - The mechanisms of sister chromatid exchanges (SCEs) are not known. One hypothesis is that SCE is a manifestation of Rad51-dependent homologous recombination repair. In order to test this hypothesis, we have compared the frequencies of SCEs induced by mitomycin C (MMC) and 254 nm ultraviolet radiation (UVC) in wt V79B and the Rad51C-deficient CL-V4B cells. SCEs were analysed in the first (M1) and second (M2) post-treatment mitoses. In M1 MMC induced the same frequencies of SCEs in CL-V4B and V79B cells, while the UVC-induced SCE frequencies were lower in CL-V4B than V79B cells. In CL-V4B cells, MMC-induced SCEs were higher in M2 than in M1, suggesting that interstrand cross-links (ICL) are either not removed completely or are transformed into another form of DNA damage that persists until the next cell cycle. We suggest that SCEs may represent a mechanism to bypass MMC-induced ICL without their removal.

KW - Bromodeoxyuridine

KW - Homologous recombination

KW - Mitomycin C

KW - Rad51C

KW - Sister chromatid exchanges

KW - UV radiation

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JO - Biochemical and biophysical research communications

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ER -

Rad51C-deficient CL-V4B cells exhibit normal levels of mitomycin C-induced SCEs but reduced levels of UVC-induced SCEs

Abstract

Keywords

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