TY - GEN
T1 - Radiofrequency circuit design and performance evaluation for small animal frequency-domain NIR fluorescence optical tomography
AU - Darne, Chinmay
AU - Zhu, Banghe
AU - Lu, Yujie
AU - Tan, I. Chih
AU - Rasmussen, John
AU - Sevick-Muraca, Eva M.
PY - 2011
Y1 - 2011
N2 - Herein we report on hardware development and evaluation for frequency-domain photon migration (FDPM) technique that is miniaturized for incorporation into a micro-CT gantry for hybrid CT/NIR/PET imaging. Immunity to endogenous optical properties and enhanced contrast associated with fluorophore lifetime is inherent to the FDPM measurements and enables unique opportunities for quantitative tomography when compared to the time independent (continuous wave) approach. A miniaturized radiofrequency (rf) circuitry has been developed in our laboratory for homodyne FDPM measurements that makes use of a single 100MHz oscillator to simultaneously launch optically modulated excitation light into a small animal as well as to modulate an NIR sensitive image intensifier for collection of fluorescent signals. The use of a single oscillator not only eliminates signal drift that otherwise results from the use of multiple oscillators individually driving both source and detector, but also reduces the circuit footprint for incorporation into the CT gantry. Herein, overall system performance parameters of signal-to-noise ratio, measurement precision, spatial resolution, modulation depth (ac/dc), excitation light rejection, and clinically relevant data acquisition times are presented for mouse phantom data. Image reconstruction of phantom data and integration of circuitry for hybrid CT/NIR/PET imaging is also presented towards the ultimate validation of NIR optical tomography using PET imaging as a "gold-standard" for quantification.
AB - Herein we report on hardware development and evaluation for frequency-domain photon migration (FDPM) technique that is miniaturized for incorporation into a micro-CT gantry for hybrid CT/NIR/PET imaging. Immunity to endogenous optical properties and enhanced contrast associated with fluorophore lifetime is inherent to the FDPM measurements and enables unique opportunities for quantitative tomography when compared to the time independent (continuous wave) approach. A miniaturized radiofrequency (rf) circuitry has been developed in our laboratory for homodyne FDPM measurements that makes use of a single 100MHz oscillator to simultaneously launch optically modulated excitation light into a small animal as well as to modulate an NIR sensitive image intensifier for collection of fluorescent signals. The use of a single oscillator not only eliminates signal drift that otherwise results from the use of multiple oscillators individually driving both source and detector, but also reduces the circuit footprint for incorporation into the CT gantry. Herein, overall system performance parameters of signal-to-noise ratio, measurement precision, spatial resolution, modulation depth (ac/dc), excitation light rejection, and clinically relevant data acquisition times are presented for mouse phantom data. Image reconstruction of phantom data and integration of circuitry for hybrid CT/NIR/PET imaging is also presented towards the ultimate validation of NIR optical tomography using PET imaging as a "gold-standard" for quantification.
KW - fluorescence
KW - frequency-domain photon migration
KW - near infrared imaging
KW - optical tomography
KW - radiofrequency
KW - small animal imaging
UR - http://www.scopus.com/inward/record.url?scp=79955717565&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79955717565&partnerID=8YFLogxK
U2 - 10.1117/12.874946
DO - 10.1117/12.874946
M3 - Conference contribution
AN - SCOPUS:79955717565
SN - 9780819484338
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Optical Tomography and Spectroscopy of Tissue IX
T2 - Optical Tomography and Spectroscopy of Tissue IX
Y2 - 23 January 2011 through 26 January 2011
ER -