Radioiodinated antibody targeting of the HER-2/neu oncoprotein

F. J. Xu; Y. H. Yu; D. S. Bae; X. G. Zhao; S. K. Slade; C. M. Boyer; Robert C. Bast; M. R. Zalutsky

doi:10.1016/S0969-8051(97)80014-5

Radioiodinated antibody targeting of the HER-2/neu oncoprotein

F. J. Xu, Y. H. Yu, D. S. Bae, X. G. Zhao, S. K. Slade, C. M. Boyer, Robert C. Bast, M. R. Zalutsky

Experimental Therapeutics

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

The HER-2/neu oncogene encodes a 185 kDa phosphoglycoprotein that is overexpressed in breast, ovarian and other cancers. Seven monoclonal antibodies reactive with this oncoprotein were labeled with ¹³¹I. In vitro experiments with SKOv3 9002-18 cells determined binding affinity, internalization and degradation. The biodistribution of these antibodies in comparison to ¹²⁵I-labeled nonspecific antibody was measured in athymic mice with SKOv3 9002-18 ovarian carcinoma xenografts. Antibody 520C9 exhibited the highest and most specific retention in tumor, peaking at 17.4 ± 5.6% ID/g at 24 h.

Original language	English (US)
Pages (from-to)	451-459
Number of pages	9
Journal	Nuclear Medicine and Biology
Volume	24
Issue number	5
DOIs	https://doi.org/10.1016/S0969-8051(97)80014-5
State	Published - Jul 1997

Keywords

C-erbB-2 (HER-2/neu)
Monoclonal antibody
Ovarian cancer
Radioiodination
Tumor

ASJC Scopus subject areas

Molecular Medicine
Radiology Nuclear Medicine and imaging
Cancer Research

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10.1016/S0969-8051(97)80014-5

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title = "Radioiodinated antibody targeting of the HER-2/neu oncoprotein",

abstract = "The HER-2/neu oncogene encodes a 185 kDa phosphoglycoprotein that is overexpressed in breast, ovarian and other cancers. Seven monoclonal antibodies reactive with this oncoprotein were labeled with 131I. In vitro experiments with SKOv3 9002-18 cells determined binding affinity, internalization and degradation. The biodistribution of these antibodies in comparison to 125I-labeled nonspecific antibody was measured in athymic mice with SKOv3 9002-18 ovarian carcinoma xenografts. Antibody 520C9 exhibited the highest and most specific retention in tumor, peaking at 17.4 ± 5.6% ID/g at 24 h.",

keywords = "C-erbB-2 (HER-2/neu), Monoclonal antibody, Ovarian cancer, Radioiodination, Tumor",

author = "Xu, {F. J.} and Yu, {Y. H.} and Bae, {D. S.} and Zhao, {X. G.} and Slade, {S. K.} and Boyer, {C. M.} and Bast, {Robert C.} and Zalutsky, {M. R.}",

note = "Funding Information: This work was supported in part by grants CA 39930 and CA 42324 from the National Cancer Institute.",

year = "1997",

month = jul,

doi = "10.1016/S0969-8051(97)80014-5",

language = "English (US)",

volume = "24",

pages = "451--459",

journal = "Nuclear Medicine and Biology",

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TY - JOUR

T1 - Radioiodinated antibody targeting of the HER-2/neu oncoprotein

AU - Xu, F. J.

AU - Yu, Y. H.

AU - Bae, D. S.

AU - Zhao, X. G.

AU - Slade, S. K.

AU - Boyer, C. M.

AU - Bast, Robert C.

AU - Zalutsky, M. R.

N1 - Funding Information: This work was supported in part by grants CA 39930 and CA 42324 from the National Cancer Institute.

PY - 1997/7

Y1 - 1997/7

N2 - The HER-2/neu oncogene encodes a 185 kDa phosphoglycoprotein that is overexpressed in breast, ovarian and other cancers. Seven monoclonal antibodies reactive with this oncoprotein were labeled with 131I. In vitro experiments with SKOv3 9002-18 cells determined binding affinity, internalization and degradation. The biodistribution of these antibodies in comparison to 125I-labeled nonspecific antibody was measured in athymic mice with SKOv3 9002-18 ovarian carcinoma xenografts. Antibody 520C9 exhibited the highest and most specific retention in tumor, peaking at 17.4 ± 5.6% ID/g at 24 h.

AB - The HER-2/neu oncogene encodes a 185 kDa phosphoglycoprotein that is overexpressed in breast, ovarian and other cancers. Seven monoclonal antibodies reactive with this oncoprotein were labeled with 131I. In vitro experiments with SKOv3 9002-18 cells determined binding affinity, internalization and degradation. The biodistribution of these antibodies in comparison to 125I-labeled nonspecific antibody was measured in athymic mice with SKOv3 9002-18 ovarian carcinoma xenografts. Antibody 520C9 exhibited the highest and most specific retention in tumor, peaking at 17.4 ± 5.6% ID/g at 24 h.

KW - C-erbB-2 (HER-2/neu)

KW - Monoclonal antibody

KW - Ovarian cancer

KW - Radioiodination

KW - Tumor

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AN - SCOPUS:0030836020

SN - 0969-8051

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EP - 459

JO - Nuclear Medicine and Biology

JF - Nuclear Medicine and Biology

IS - 5

ER -

Radioiodinated antibody targeting of the HER-2/neu oncoprotein

Abstract

Keywords

ASJC Scopus subject areas

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