TY - JOUR
T1 - Radioprotection by Podophyllum hexandrum in the liver of mice
T2 - A mechanistic approach
AU - Kumar, Raj
AU - Singh, Pankaj Kumar
AU - Arora, Rajesh
AU - Sharma, Ashok
AU - Prasad, Jagdish
AU - Sagar, Ravinder
AU - Singh, Surendar
AU - Sharma, Rakesh Kumar
N1 - Funding Information:
This work was supported by a grant provided by Defence Research and Development Organization (DRDO), Ministry of Defence, Government of India. Financial support in the form of a research fellowship awarded to Mr. Pankaj Kumar Singh by Indian Council of Medical Research (ICMR) is duly acknowledged.
PY - 2005/9
Y1 - 2005/9
N2 - To evaluate radiation protection offered by the extract of Podophyllum hexandrum, expression of various cytoprotective proteins was studied using liver of Swiss albino Strain 'A' male mouse by immunoblotting. Induction of heat-shock factor-1 (HSF-1), led to up-regulation of heat-shock protein-70 (HSP-70) upon P. hexandrum (200 mg/kg b. wt.; i.p. 50% ethanolic extract) treatment 2 h before irradiation (10 Gy) as compared to sham-irradiated control. Translocation of free nuclear factor kappa B (NFκB) from cytoplasm to nucleus was found to be inhibited upon P. hexandrum treatment. An increase in Bcl-2, proliferating cell nuclear antigen (PCNA) along with a decrease in p53, caspase-3, apoptosis inducing factor (AIF) expression was observed in the mice treated with P. hexandrum. The present study indicated that P. hexandrum extract provides protection from radiation by modulation of expression of the proteins associated with apoptosis.
AB - To evaluate radiation protection offered by the extract of Podophyllum hexandrum, expression of various cytoprotective proteins was studied using liver of Swiss albino Strain 'A' male mouse by immunoblotting. Induction of heat-shock factor-1 (HSF-1), led to up-regulation of heat-shock protein-70 (HSP-70) upon P. hexandrum (200 mg/kg b. wt.; i.p. 50% ethanolic extract) treatment 2 h before irradiation (10 Gy) as compared to sham-irradiated control. Translocation of free nuclear factor kappa B (NFκB) from cytoplasm to nucleus was found to be inhibited upon P. hexandrum treatment. An increase in Bcl-2, proliferating cell nuclear antigen (PCNA) along with a decrease in p53, caspase-3, apoptosis inducing factor (AIF) expression was observed in the mice treated with P. hexandrum. The present study indicated that P. hexandrum extract provides protection from radiation by modulation of expression of the proteins associated with apoptosis.
KW - Apoptosis
KW - DNA protection
KW - Gamma radiation
KW - Podophyllum hexandrum
KW - Radioprotection
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U2 - 10.1016/j.etap.2005.03.008
DO - 10.1016/j.etap.2005.03.008
M3 - Article
C2 - 21783608
AN - SCOPUS:23044441572
SN - 1382-6689
VL - 20
SP - 326
EP - 334
JO - Environmental Toxicology and Pharmacology
JF - Environmental Toxicology and Pharmacology
IS - 2
ER -