Abstract
Introduction: The hepatocellular carcinoma-intestine-pancreas and pancreatitis-associated proteins, also known as lactose-binding protein, is upregulated in peritumoral pancreatic tissue. Previously, we reported ethyl-β-D-galactopyranosyl-(1,4′)-2′-deoxy-2′-[ 18F]fluoro-β-D-glucopyranoside (Et-[18F]-FDL), a radiofluorinated lactose analog for positron emission tomography (PET) of small pancreatic carcinomas in mice. However, synthesis of the precursor for Et-[ 18F]-FDL involves 11 steps, which is quite lengthy, and produces overall low yields. Here, we report on synthesis and radiolabeling of another analog of lactose, the 1′-[18F]fluoroethyl-β-D-lactose for PET imaging of pancreatic carcinomas. Methods: Two precursor compounds, 1′-bromoethyl-2′,3′,6′,2,3,4,6-hepta-O-acetyl-β-D- lactose 4, and 1′-p-toluenesulfonylethyl-2′,3′,6′,2,3,4, 6-hepta-O-acetyl-β-D-lactose 5, were synthesized in two and three steps, respectively; then, cold fluorination and radiofluorination of these precursors were performed. The reaction mixture was passed through a silica gel Sep-pack cartridge, eluted with EtOAc, and the 1′-[18F]fluoroethyl- 2′,3′,6′,2,3,4,6-hepta-O-acetyl-β-D-lactose ([ 18F]-6) purified by HPLC. After hydrolysis of the protecting groups, the 1′-[18F]fluoroethyl-β-D-lactose [18F]-7 was neutralized, diluted with saline, filtered through a sterile Millipore filter, and analyzed by radio-TLC. Results: The average decay-corrected radiochemical yield was 9% (n = 7) with>99% radiochemical purity and specific activity of 55.5 GBq/μmol. Conclusion: A new analog of lactose, 1′-[ 18F]fluoroethyl-β-D-lactose, has been synthesized in good yields, with high purity and high specific activity suitable for PET imaging of early pancreatic carcinomas.
Original language | English (US) |
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Pages (from-to) | 233-238 |
Number of pages | 6 |
Journal | Journal of Labelled Compounds and Radiopharmaceuticals |
Volume | 54 |
Issue number | 5 |
DOIs | |
State | Published - May 15 2011 |
Keywords
- HIP/PAP
- PET
- fluorine-18
- lactose
- pancreatic carcinoma
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Radiology Nuclear Medicine and imaging
- Drug Discovery
- Spectroscopy
- Organic Chemistry