TY - JOUR
T1 - Reduced expression of mismatch repair genes in colorectal cancer patients in Egypt
AU - Soliman, Amr S.
AU - Bondy, Melissa L.
AU - Guan, Yongli
AU - El-Badawi, Sami
AU - Mokhtar, Nadia
AU - Bayomi, Sherif
AU - Raouf, Ahmed A.
AU - Ismail, Sohair
AU - McPherson, R. Sue
AU - Abdel-Hakim, Tarek F.
AU - Beasley, R. Palmer
AU - Levin, Bernard
AU - Wei, Qingyi
N1 - Funding Information:
Funding for this project was provided by the United States Agency for International Development (USAID). This research has been supported in part by the President’s Emergency Plan for AIDS Relief (PEPFAR) through the U.S. Centers for Disease Control and Prevention.
PY - 1998/6
Y1 - 1998/6
N2 - An Egyptian hospital-based pilot case-control study was conducted to investigate the relationship between the expression level of mismatch repair (MMR) genes and the risk of colorectal cancer. The relative expression of five known MMR genes, i.e., hMSH2, hMLH1, hPMS1, hPMS2, and GTBP/hMSH6, was measured by a multiplex reverse transcriptase (RT)-polymerase chain reaction (PCR) in peripheral blood lymphocytes from 31 colorectal cancer patients and 47 age- and-sex matched controls. The expression of hMSH2, GTBP/hMSH6, hPMS1 and hPMS2 tended to be lower in patients than controls, but only the difference in hPMS2 expression was statistically significant (p<0.01). Although 50% of the cases had chemotherapy or radiotherapy within the last Six months before the blood was drawn, their gene expression was not statistically different from those who had not Undergone such therapies. After adjustment for age and sex, the odds ratios (OR) calculated from a logistical regression model, using the median levels of gene expression of controls as cut-off values, indicated that increased risk was associated with reduced expressions of both hPMS1 (OR = 3.97, 95% confidence interval (CI) = 1.04 to 7.65) and hPMS2 (OR = 2.86, 95% CI = 1.05 to 7.76). Although the results of this study were inconclusive because of the small sample size and use of prevalent cases, it is biologically plausible that patients with colorectal cancers may have a lower expression of MMR genes than healthy controls because malfunction of these genes has been shown in hereditary nonpolyposis colon cancer. The involvement of low hPMS2 expression in colon cancer risk seems to be unique in the Egyptian population. Further studies with newly diagnosed patients before they begin therapy will provide more convincing data about the role of MMR gene expression in the etiology of colorectal cancers in Egypt.
AB - An Egyptian hospital-based pilot case-control study was conducted to investigate the relationship between the expression level of mismatch repair (MMR) genes and the risk of colorectal cancer. The relative expression of five known MMR genes, i.e., hMSH2, hMLH1, hPMS1, hPMS2, and GTBP/hMSH6, was measured by a multiplex reverse transcriptase (RT)-polymerase chain reaction (PCR) in peripheral blood lymphocytes from 31 colorectal cancer patients and 47 age- and-sex matched controls. The expression of hMSH2, GTBP/hMSH6, hPMS1 and hPMS2 tended to be lower in patients than controls, but only the difference in hPMS2 expression was statistically significant (p<0.01). Although 50% of the cases had chemotherapy or radiotherapy within the last Six months before the blood was drawn, their gene expression was not statistically different from those who had not Undergone such therapies. After adjustment for age and sex, the odds ratios (OR) calculated from a logistical regression model, using the median levels of gene expression of controls as cut-off values, indicated that increased risk was associated with reduced expressions of both hPMS1 (OR = 3.97, 95% confidence interval (CI) = 1.04 to 7.65) and hPMS2 (OR = 2.86, 95% CI = 1.05 to 7.76). Although the results of this study were inconclusive because of the small sample size and use of prevalent cases, it is biologically plausible that patients with colorectal cancers may have a lower expression of MMR genes than healthy controls because malfunction of these genes has been shown in hereditary nonpolyposis colon cancer. The involvement of low hPMS2 expression in colon cancer risk seems to be unique in the Egyptian population. Further studies with newly diagnosed patients before they begin therapy will provide more convincing data about the role of MMR gene expression in the etiology of colorectal cancers in Egypt.
KW - Colorectal cancer
KW - Gene expression
KW - Mismatch repair
KW - Polymerase chain reaction
KW - Reverse transcription
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U2 - 10.3892/ijo.12.6.1315
DO - 10.3892/ijo.12.6.1315
M3 - Article
C2 - 9592192
AN - SCOPUS:13144256740
SN - 1019-6439
VL - 12
SP - 1315
EP - 1319
JO - International journal of oncology
JF - International journal of oncology
IS - 6
ER -