Abstract
Refolding of recombinant proteins is an important event in the downstream process of genetic engineering. On the basis of the mechanism of protein folding in vitro, general strategy of recombinant protein refolding is postulated, and major developments in recent years on this field are reviewed, including: molecular chaperone mediated refolding, detergent-assisted refolding, protein refolding in reverse micelle, addition of folding enhancer and methods for the formation of disulfide bond during protein refolding.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 103-108 |
| Number of pages | 6 |
| Journal | Sheng li ke xue jin zhan [Progress in physiology] |
| Volume | 29 |
| Issue number | 2 |
| State | Published - Apr 1998 |
| Externally published | Yes |
ASJC Scopus subject areas
- General Medicine