Regional mapping of human DNA excision repair gene ERCC4 to chromosome 16pl3.13-pl3.2

P. Liu; J. Siciliano; B. White; R. Legerski; D. Callen; S. Reeders; M. J. Siciliano; L. H. Thompson

doi:10.1093/mutage/8.3.199

Regional mapping of human DNA excision repair gene ERCC4 to chromosome 16pl3.13-pl3.2

P. Liu, J. Siciliano, B. White, R. Legerski, D. Callen, S. Reeders, M. J. Siciliano, L. H. Thompson

Genetics

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Mitomycin C (MMC)-resistant interspecific somatic cell hybrids made between human cells and the MMC-sensitive, Chinese hamster ovary (CHO) excision repair-deficient UV41 cells generally contained human chromosome 16, while other human chromosomes were randomly present. MMC-sensitive and -resistant subclones were isolated from resistant clones and resistance generally segregated concordantly with human chromosome 16 markers. UV radiation survival analysis of subclones indicated that MMC and UV resistance were correlated. Therefore the complementing gene, Excision Repair Cross Complementing 4 (ERCC4) was assigned to human chromosome 16. Complementation of UV41 by human cells derived from patients with xeroderma pigmentosum groups A, C, D and F excluded ERCC4 from involvement in those disease syndromes. Resistant hybrids containing only portions of chromosome 16 were identified by the lack of concordance of multiple chromosome 16 markers. When such hybrids were used as a source of probe for fluorescent in situ hybridization onto normal human metaphases, the only region of chromosome 16 identified as being consistently present was 16pl3.1-pl3.3. Genetic marker analysis of informative hybrids with mapped probes refined the position of ERCC4 to 16pl3.13-pl3.2 and allowed the following order of markers within the region to be established: pter-(PRM1 D16S215)-D16S213-D16S53 -(D16S214,ERCC4)-D16S3-D16S96-cen

Original language	English (US)
Pages (from-to)	199-205
Number of pages	7
Journal	Mutagenesis
Volume	8
Issue number	3
DOIs	https://doi.org/10.1093/mutage/8.3.199
State	Published - May 1993

ASJC Scopus subject areas

Genetics
Toxicology
Genetics(clinical)
Health, Toxicology and Mutagenesis

Access to Document

10.1093/mutage/8.3.199

Cite this

@article{a73c79c93d1a430ab8866e36a7a88edd,

title = "Regional mapping of human DNA excision repair gene ERCC4 to chromosome 16pl3.13-pl3.2",

abstract = "Mitomycin C (MMC)-resistant interspecific somatic cell hybrids made between human cells and the MMC-sensitive, Chinese hamster ovary (CHO) excision repair-deficient UV41 cells generally contained human chromosome 16, while other human chromosomes were randomly present. MMC-sensitive and -resistant subclones were isolated from resistant clones and resistance generally segregated concordantly with human chromosome 16 markers. UV radiation survival analysis of subclones indicated that MMC and UV resistance were correlated. Therefore the complementing gene, Excision Repair Cross Complementing 4 (ERCC4) was assigned to human chromosome 16. Complementation of UV41 by human cells derived from patients with xeroderma pigmentosum groups A, C, D and F excluded ERCC4 from involvement in those disease syndromes. Resistant hybrids containing only portions of chromosome 16 were identified by the lack of concordance of multiple chromosome 16 markers. When such hybrids were used as a source of probe for fluorescent in situ hybridization onto normal human metaphases, the only region of chromosome 16 identified as being consistently present was 16pl3.1-pl3.3. Genetic marker analysis of informative hybrids with mapped probes refined the position of ERCC4 to 16pl3.13-pl3.2 and allowed the following order of markers within the region to be established: pter-(PRM1 D16S215)-D16S213-D16S53 -(D16S214,ERCC4)-D16S3-D16S96-cen",

author = "P. Liu and J. Siciliano and B. White and R. Legerski and D. Callen and S. Reeders and Siciliano, {M. J.} and Thompson, {L. H.}",

note = "Funding Information: Supported in part by NIH grants CA34936, CA04484 and CA55164, and a gift from Mr Kenneth D.Muller. A portion of this work was performed under the auspices of the US Department of Energy by the Lawrence Livermore National Laboratory under contract no. W-7405-ENG-48.",

year = "1993",

month = may,

doi = "10.1093/mutage/8.3.199",

language = "English (US)",

volume = "8",

pages = "199--205",

journal = "Mutagenesis",

issn = "0267-8357",

publisher = "Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Regional mapping of human DNA excision repair gene ERCC4 to chromosome 16pl3.13-pl3.2

AU - Liu, P.

AU - Siciliano, J.

AU - White, B.

AU - Legerski, R.

AU - Callen, D.

AU - Reeders, S.

AU - Siciliano, M. J.

AU - Thompson, L. H.

N1 - Funding Information: Supported in part by NIH grants CA34936, CA04484 and CA55164, and a gift from Mr Kenneth D.Muller. A portion of this work was performed under the auspices of the US Department of Energy by the Lawrence Livermore National Laboratory under contract no. W-7405-ENG-48.

PY - 1993/5

Y1 - 1993/5

N2 - Mitomycin C (MMC)-resistant interspecific somatic cell hybrids made between human cells and the MMC-sensitive, Chinese hamster ovary (CHO) excision repair-deficient UV41 cells generally contained human chromosome 16, while other human chromosomes were randomly present. MMC-sensitive and -resistant subclones were isolated from resistant clones and resistance generally segregated concordantly with human chromosome 16 markers. UV radiation survival analysis of subclones indicated that MMC and UV resistance were correlated. Therefore the complementing gene, Excision Repair Cross Complementing 4 (ERCC4) was assigned to human chromosome 16. Complementation of UV41 by human cells derived from patients with xeroderma pigmentosum groups A, C, D and F excluded ERCC4 from involvement in those disease syndromes. Resistant hybrids containing only portions of chromosome 16 were identified by the lack of concordance of multiple chromosome 16 markers. When such hybrids were used as a source of probe for fluorescent in situ hybridization onto normal human metaphases, the only region of chromosome 16 identified as being consistently present was 16pl3.1-pl3.3. Genetic marker analysis of informative hybrids with mapped probes refined the position of ERCC4 to 16pl3.13-pl3.2 and allowed the following order of markers within the region to be established: pter-(PRM1 D16S215)-D16S213-D16S53 -(D16S214,ERCC4)-D16S3-D16S96-cen

AB - Mitomycin C (MMC)-resistant interspecific somatic cell hybrids made between human cells and the MMC-sensitive, Chinese hamster ovary (CHO) excision repair-deficient UV41 cells generally contained human chromosome 16, while other human chromosomes were randomly present. MMC-sensitive and -resistant subclones were isolated from resistant clones and resistance generally segregated concordantly with human chromosome 16 markers. UV radiation survival analysis of subclones indicated that MMC and UV resistance were correlated. Therefore the complementing gene, Excision Repair Cross Complementing 4 (ERCC4) was assigned to human chromosome 16. Complementation of UV41 by human cells derived from patients with xeroderma pigmentosum groups A, C, D and F excluded ERCC4 from involvement in those disease syndromes. Resistant hybrids containing only portions of chromosome 16 were identified by the lack of concordance of multiple chromosome 16 markers. When such hybrids were used as a source of probe for fluorescent in situ hybridization onto normal human metaphases, the only region of chromosome 16 identified as being consistently present was 16pl3.1-pl3.3. Genetic marker analysis of informative hybrids with mapped probes refined the position of ERCC4 to 16pl3.13-pl3.2 and allowed the following order of markers within the region to be established: pter-(PRM1 D16S215)-D16S213-D16S53 -(D16S214,ERCC4)-D16S3-D16S96-cen

UR - http://www.scopus.com/inward/record.url?scp=0027207298&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027207298&partnerID=8YFLogxK

U2 - 10.1093/mutage/8.3.199

DO - 10.1093/mutage/8.3.199

M3 - Article

C2 - 8332082

AN - SCOPUS:0027207298

SN - 0267-8357

VL - 8

SP - 199

EP - 205

JO - Mutagenesis

JF - Mutagenesis

IS - 3

ER -

Regional mapping of human DNA excision repair gene ERCC4 to chromosome 16pl3.13-pl3.2

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this