@article{c30e23fef9b849b594340c8024733727,
title = "Regulation of telomerase during human placental differentiation: A role for TGFβ1",
abstract = "The transient tumor-like attributes of the first-trimester placenta anchor the developing embryo to the uterine wall thus establishing a vital link between the mother and the fetus. Dysregulation of this invasive behavior and/or controlled proliferation of the placenta is associated with abnormal pregnancies. Several of these diseased states also exhibit aberrant telomerase activity, among other pathophysiological manifestations. Considering the strong correlation between telomerase activity and tumorigenesis, it was of interest to see whether the crucial processes of trophoblast proliferation and differentiation were brought about through the modulation of telomerase. Using two in vitro model systems of trophoblast differentiation, we demonstrate here that telomerase activity is negatively regulated during placental differentiation. We further show that this modulation is at the level of transcription of hTERT. We also propose a role for TGF β1 in regulating telomerase activity in differentiating trophoblasts by down-regulating the expression of hTERT at the transcriptional level.",
keywords = "Differentiation, Placenta, Proliferation, Telomerase, Trophoblast",
author = "S. Rama and Y. Suresh and Rao, {A. J.}",
note = "Funding Information: We are extremely grateful to Dr Satoru Kyo, Department of Obstetrics and Gynecology, Kanazawa University, Japan, for extensive help in establishing the TRAP assay in our laboratory and to Dr Susan Fisher, Department of Stomatology, University of California, San Francisco, California, for her kind gift of the BeWo cell line. We thank Dr Peter Petrusz, Laboratories for Reproductive Biology (LRB) and Department of Cell and Developmental Biology, University of North Carolina, Chapel Hill, for generously allowing us to use the immunohistochemistry core facility and also for his expert advice. Thanks to Ms Gail Grossman, Department of Cell and Developmental Biology and LRB, University of North Carolina, Chapel Hill, for guidance and help with the hTERT immuno-staining. We thank Dr P K Lala, Department of Anatomy, University of Western Ontario, Canada, Prof A M Dharmarajan, Department of Anatomy and Human Biology, University of Western Australia, Perth, Dr P Kondaiah, Department of MRDG, IISc and Dr Anjali Karande, Department of Biochemistry, IISc for their helpful suggestions. Thanks to Suchithra and Padmini for expert technical assistance. We acknowledge the help provided by the DNA sequencing facility, IISc, Bangalore. We also thank the medical and supporting staff of Lakshmi Maternity Home, Manipal Northside Hospital and Desai Nursing Home, Bangalore, for their help in collection of human placental tissue. This study is financially supported by the Council for Scientific and Industrial Research & Department of Biotechnology, Government of India. ",
year = "2001",
month = sep,
day = "20",
doi = "10.1016/S0303-7207(01)00550-0",
language = "English (US)",
volume = "182",
pages = "233--248",
journal = "Molecular and cellular endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",
number = "2",
}