Rejection of dinitrochlorobenzene-conjugated syngeneic tumor cells by dinitrochlorobenzene-sensitized guinea pigs

J. A. Roth; D. L. Morton; E. C. Holmes

doi:10.1016/0022-4804(78)90148-8

Rejection of dinitrochlorobenzene-conjugated syngeneic tumor cells by dinitrochlorobenzene-sensitized guinea pigs

J. A. Roth, D. L. Morton, E. C. Holmes

Thoracic & Cardiovascular Surgery

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Dinitrophenylation of tumor cells was investigated as a method for augmenting tumor cell antigenicity in the DNCB-sensitized host. Strain-2 guinea pigs were hyper-immunized to DNCB by intradermal injection. A syngeneic methylcholanthrene-induced sarcoma (MCA-25), was conjugated to DNCB without significant loss of viability. DNCB-Sensitized animals and unsensitized animals were injected intradermally on opposite flanks with conjugated and unconjugated tumor cells. DNCB-Conjugated tumor cells produced significantly fewer tumors in DNCB-sensitized animals (8/21) when compared to unsensitized animals (17/22). The growth of unconjugated tumor cells was significantly inhibited by the simultaneous injection of conjugated tumor cells in DNCB-sensitized animals. DNCB sensitization did not affect tumor growth in a control group receiving unconjugated tumor cells. DNCB sensitization, therefore, will induce immunity to the hapten-tumor cell conjugate.

Original language	English (US)
Pages (from-to)	1-7
Number of pages	7
Journal	Journal of Surgical Research
Volume	25
Issue number	1
DOIs	https://doi.org/10.1016/0022-4804(78)90148-8
State	Published - Jul 1978

ASJC Scopus subject areas

Surgery

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10.1016/0022-4804(78)90148-8

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@article{d95fca488bce4fb68925fc1df06d37e3,

title = "Rejection of dinitrochlorobenzene-conjugated syngeneic tumor cells by dinitrochlorobenzene-sensitized guinea pigs",

abstract = "Dinitrophenylation of tumor cells was investigated as a method for augmenting tumor cell antigenicity in the DNCB-sensitized host. Strain-2 guinea pigs were hyper-immunized to DNCB by intradermal injection. A syngeneic methylcholanthrene-induced sarcoma (MCA-25), was conjugated to DNCB without significant loss of viability. DNCB-Sensitized animals and unsensitized animals were injected intradermally on opposite flanks with conjugated and unconjugated tumor cells. DNCB-Conjugated tumor cells produced significantly fewer tumors in DNCB-sensitized animals (8/21) when compared to unsensitized animals (17/22). The growth of unconjugated tumor cells was significantly inhibited by the simultaneous injection of conjugated tumor cells in DNCB-sensitized animals. DNCB sensitization did not affect tumor growth in a control group receiving unconjugated tumor cells. DNCB sensitization, therefore, will induce immunity to the hapten-tumor cell conjugate.",

author = "Roth, {J. A.} and Morton, {D. L.} and Holmes, {E. C.}",

note = "Funding Information: {\textquoteright} This study was supported CA12582, CA05262, and CA02285, tional Cancer Institute (DHEW), search Service of the Veterans",

year = "1978",

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doi = "10.1016/0022-4804(78)90148-8",

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AU - Roth, J. A.

AU - Morton, D. L.

AU - Holmes, E. C.

N1 - Funding Information: ’ This study was supported CA12582, CA05262, and CA02285, tional Cancer Institute (DHEW), search Service of the Veterans

PY - 1978/7

Y1 - 1978/7

N2 - Dinitrophenylation of tumor cells was investigated as a method for augmenting tumor cell antigenicity in the DNCB-sensitized host. Strain-2 guinea pigs were hyper-immunized to DNCB by intradermal injection. A syngeneic methylcholanthrene-induced sarcoma (MCA-25), was conjugated to DNCB without significant loss of viability. DNCB-Sensitized animals and unsensitized animals were injected intradermally on opposite flanks with conjugated and unconjugated tumor cells. DNCB-Conjugated tumor cells produced significantly fewer tumors in DNCB-sensitized animals (8/21) when compared to unsensitized animals (17/22). The growth of unconjugated tumor cells was significantly inhibited by the simultaneous injection of conjugated tumor cells in DNCB-sensitized animals. DNCB sensitization did not affect tumor growth in a control group receiving unconjugated tumor cells. DNCB sensitization, therefore, will induce immunity to the hapten-tumor cell conjugate.

AB - Dinitrophenylation of tumor cells was investigated as a method for augmenting tumor cell antigenicity in the DNCB-sensitized host. Strain-2 guinea pigs were hyper-immunized to DNCB by intradermal injection. A syngeneic methylcholanthrene-induced sarcoma (MCA-25), was conjugated to DNCB without significant loss of viability. DNCB-Sensitized animals and unsensitized animals were injected intradermally on opposite flanks with conjugated and unconjugated tumor cells. DNCB-Conjugated tumor cells produced significantly fewer tumors in DNCB-sensitized animals (8/21) when compared to unsensitized animals (17/22). The growth of unconjugated tumor cells was significantly inhibited by the simultaneous injection of conjugated tumor cells in DNCB-sensitized animals. DNCB sensitization did not affect tumor growth in a control group receiving unconjugated tumor cells. DNCB sensitization, therefore, will induce immunity to the hapten-tumor cell conjugate.

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Rejection of dinitrochlorobenzene-conjugated syngeneic tumor cells by dinitrochlorobenzene-sensitized guinea pigs

Abstract

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