TY - JOUR
T1 - Repair of UV light-induced DNA damage and risk of cutaneous malignant melanoma
AU - Wei, Qingyi
AU - Lee, Jeffrey E.
AU - Gershenwald, Jeffrey E.
AU - Ross, Merrick I.
AU - Mansfield, Paul F.
AU - Strom, Sara S.
AU - Wang, Li E.
AU - Guo, Zhaozheng
AU - Qiao, Yawei
AU - Amos, Christopher I.
AU - Spitz, Margaret R.
AU - Duvic, Madeline
PY - 2003/2/19
Y1 - 2003/2/19
N2 - Background: The mechanism underlying the role of UV light exposure from sunlight in the etiology of cutaneous malignant melanoma (CMM) is unclear. Patients with xeroderma pigmentosum, a disease characterized by severe sensitivity to UV radiation and a defect in nucleotide excision repair, have a high incidence of CMM, which suggests that DNA repair capacity (DRC) plays a role in sunlight-induced CMM in the general population as well. Methods: We conducted a hospital-based case-control study of DRC and CMM among 312 non-Hispanic white CMM patients who had no prior chemotherapy or radiation therapy, and 324 cancer-free control subjects who were frequency-matched to case patients on age, sex, and ethnicity. Information on demographic variables, risk factors, and tumor characteristics was obtained from questionnaires and medical records. We used the host-cell reactivation assay to measure the DRC in study subjects' lymphocytes. All statistical tests were two sided. Results: Case patients had a 19% lower mean (± standard deviation [SD]) DRC (8.5 ± 3.4%) than control subjects (10.5 ± 5.1%), a statistically significant difference (P<.001). DRC that was at or below the median value (i.e., 9.4%) in control subjects was associated with increased risk for CMM after adjustment for age, sex, and other covariates (odds ratio [OR] = 2.02, 95% confidence interval [CI] = 1.45 to 2.82). We observed a dose-response relationship between decreased DRC and increased risk of CMM (Ptrend<.001). Patients with tumors on sun-exposed skin had statistically significantly lower DRC than patients with tumors on unexposed skin (8.2 ± 3.3% versus 9.5 ± 3.5%; P = .004). Conclusions: Reduced DRC is an independent risk factor for CMM and may contribute to susceptibility to sunlight-induced CMM among the general population.
AB - Background: The mechanism underlying the role of UV light exposure from sunlight in the etiology of cutaneous malignant melanoma (CMM) is unclear. Patients with xeroderma pigmentosum, a disease characterized by severe sensitivity to UV radiation and a defect in nucleotide excision repair, have a high incidence of CMM, which suggests that DNA repair capacity (DRC) plays a role in sunlight-induced CMM in the general population as well. Methods: We conducted a hospital-based case-control study of DRC and CMM among 312 non-Hispanic white CMM patients who had no prior chemotherapy or radiation therapy, and 324 cancer-free control subjects who were frequency-matched to case patients on age, sex, and ethnicity. Information on demographic variables, risk factors, and tumor characteristics was obtained from questionnaires and medical records. We used the host-cell reactivation assay to measure the DRC in study subjects' lymphocytes. All statistical tests were two sided. Results: Case patients had a 19% lower mean (± standard deviation [SD]) DRC (8.5 ± 3.4%) than control subjects (10.5 ± 5.1%), a statistically significant difference (P<.001). DRC that was at or below the median value (i.e., 9.4%) in control subjects was associated with increased risk for CMM after adjustment for age, sex, and other covariates (odds ratio [OR] = 2.02, 95% confidence interval [CI] = 1.45 to 2.82). We observed a dose-response relationship between decreased DRC and increased risk of CMM (Ptrend<.001). Patients with tumors on sun-exposed skin had statistically significantly lower DRC than patients with tumors on unexposed skin (8.2 ± 3.3% versus 9.5 ± 3.5%; P = .004). Conclusions: Reduced DRC is an independent risk factor for CMM and may contribute to susceptibility to sunlight-induced CMM among the general population.
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U2 - 10.1093/jnci/95.4.308
DO - 10.1093/jnci/95.4.308
M3 - Article
C2 - 12591987
AN - SCOPUS:0037453898
SN - 0027-8874
VL - 95
SP - 308
EP - 315
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 4
ER -