Repetitive noninvasive monitoring of hsvl-tk-expressing t cells intravenously infused into nonhuman primates using positron emission tomography and computed tomography with 18F-FEAU

Juri G. Gelovani; Gianpietro Dotti; Mei Tian; Barbara Savoldo; Amer Najjar; Laurence Cooper; James Jackson; Amanda Smith; Osama Mawlawi; Rajesh Uthamanthil; Agatha Borne; David Brammer; Vincenzo Paolillo; Mian Alauddin; Carlos Gonzalez; David Steiner; William K. Decker; Frank Marini; Steven Kornblau; Catherine M. Bollard; Elizabeth J. Shpall

doi:10.2310/7290.2009.00022

Repetitive noninvasive monitoring of hsvl-tk-expressing t cells intravenously infused into nonhuman primates using positron emission tomography and computed tomography with ¹⁸F-FEAU

Juri G. Gelovani, Gianpietro Dotti, Mei Tian, Barbara Savoldo, Amer Najjar, Laurence Cooper, James Jackson, Amanda Smith, Osama Mawlawi, Rajesh Uthamanthil, Agatha Borne, David Brammer, Vincenzo Paolillo, Mian Alauddin, Carlos Gonzalez, David Steiner, William K. Decker, Frank Marini, Steven Kornblau, Catherine M. BollardElizabeth J. Shpall

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) has been successfully used to treat patients with different types of cancer. However, the long-term spatial-temporal dynamics of the distribution of systemically infused CTLs rlargely unknown. Noninvasive imaging of adoptively transferred CTLs using molecular-genetic reporter imaging with positron emission tomography and computed tomography (PET-CT) represents an innovative approach to understanding the long-term migratory patterns and therapeutic potential of adoptively transferred T cells. Here we report the application of repetitive PET-CT imaging with [¹⁸F]fluoro-5-ethyl-1-beta-D- arabinofuranosyluracil (¹⁸F-FEAU) in two nonhuman primates demonstrating that autologous polyclonal macaque T lymphocytes activated and transduced with a retroviral vector encoding for the sr39 mutant herpes simplex virus 1 thymidine kinase (sr39HSV1-tk) reporter gene can be detected after intravenous infusion in discrete lymphoid organs and in sites of inflammation. This study represents a proof of principle and supports the application of ¹⁸F-FEAU PET-CT imaging for monitoring the distribution of intravenously administered sr39HSV1-tk gene-transduced CTLs in humans.

Original language	English (US)
Pages (from-to)	230-237
Number of pages	8
Journal	Molecular imaging
Volume	8
Issue number	4
DOIs	https://doi.org/10.2310/7290.2009.00022
State	Published - Jul 2009

ASJC Scopus subject areas

Biotechnology
Molecular Medicine
Biomedical Engineering
Radiology Nuclear Medicine and imaging
Condensed Matter Physics

MD Anderson CCSG core facilities

Advanced Technology Genomics Core
Small Animal Imaging Facility

Access to Document

10.2310/7290.2009.00022

Cite this

Gelovani, J. G., Dotti, G., Tian, M., Savoldo, B., Najjar, A., Cooper, L., Jackson, J., Smith, A., Mawlawi, O., Uthamanthil, R., Borne, A., Brammer, D., Paolillo, V., Alauddin, M., Gonzalez, C., Steiner, D., Decker, W. K., Marini, F., Kornblau, S., ... Shpall, E. J. (2009). Repetitive noninvasive monitoring of hsvl-tk-expressing t cells intravenously infused into nonhuman primates using positron emission tomography and computed tomography with ¹⁸F-FEAU. Molecular imaging, 8(4), 230-237. https://doi.org/10.2310/7290.2009.00022

Gelovani, JG, Dotti, G, Tian, M, Savoldo, B, Najjar, A, Cooper, L, Jackson, J, Smith, A, Mawlawi, O, Uthamanthil, R, Borne, A, Brammer, D, Paolillo, V, Alauddin, M, Gonzalez, C, Steiner, D, Decker, WK, Marini, F, Kornblau, S, Bollard, CM & Shpall, EJ 2009, 'Repetitive noninvasive monitoring of hsvl-tk-expressing t cells intravenously infused into nonhuman primates using positron emission tomography and computed tomography with ¹⁸F-FEAU', Molecular imaging, vol. 8, no. 4, pp. 230-237. https://doi.org/10.2310/7290.2009.00022

@article{6b3074dbce304052b9879e84c5de8492,

title = "Repetitive noninvasive monitoring of hsvl-tk-expressing t cells intravenously infused into nonhuman primates using positron emission tomography and computed tomography with 18F-FEAU",

abstract = "Adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) has been successfully used to treat patients with different types of cancer. However, the long-term spatial-temporal dynamics of the distribution of systemically infused CTLs rlargely unknown. Noninvasive imaging of adoptively transferred CTLs using molecular-genetic reporter imaging with positron emission tomography and computed tomography (PET-CT) represents an innovative approach to understanding the long-term migratory patterns and therapeutic potential of adoptively transferred T cells. Here we report the application of repetitive PET-CT imaging with [18F]fluoro-5-ethyl-1-beta-D- arabinofuranosyluracil (18F-FEAU) in two nonhuman primates demonstrating that autologous polyclonal macaque T lymphocytes activated and transduced with a retroviral vector encoding for the sr39 mutant herpes simplex virus 1 thymidine kinase (sr39HSV1-tk) reporter gene can be detected after intravenous infusion in discrete lymphoid organs and in sites of inflammation. This study represents a proof of principle and supports the application of 18F-FEAU PET-CT imaging for monitoring the distribution of intravenously administered sr39HSV1-tk gene-transduced CTLs in humans.",

author = "Gelovani, {Juri G.} and Gianpietro Dotti and Mei Tian and Barbara Savoldo and Amer Najjar and Laurence Cooper and James Jackson and Amanda Smith and Osama Mawlawi and Rajesh Uthamanthil and Agatha Borne and David Brammer and Vincenzo Paolillo and Mian Alauddin and Carlos Gonzalez and David Steiner and Decker, {William K.} and Frank Marini and Steven Kornblau and Bollard, {Catherine M.} and Shpall, {Elizabeth J.}",

year = "2009",

month = jul,

doi = "10.2310/7290.2009.00022",

language = "English (US)",

volume = "8",

pages = "230--237",

journal = "Molecular imaging",

issn = "1535-3508",

publisher = "Decker Publishing",

number = "4",

}

TY - JOUR

T1 - Repetitive noninvasive monitoring of hsvl-tk-expressing t cells intravenously infused into nonhuman primates using positron emission tomography and computed tomography with 18F-FEAU

AU - Gelovani, Juri G.

AU - Dotti, Gianpietro

AU - Tian, Mei

AU - Savoldo, Barbara

AU - Najjar, Amer

AU - Cooper, Laurence

AU - Jackson, James

AU - Smith, Amanda

AU - Mawlawi, Osama

AU - Uthamanthil, Rajesh

AU - Borne, Agatha

AU - Brammer, David

AU - Paolillo, Vincenzo

AU - Alauddin, Mian

AU - Gonzalez, Carlos

AU - Steiner, David

AU - Decker, William K.

AU - Marini, Frank

AU - Kornblau, Steven

AU - Bollard, Catherine M.

AU - Shpall, Elizabeth J.

PY - 2009/7

Y1 - 2009/7

N2 - Adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) has been successfully used to treat patients with different types of cancer. However, the long-term spatial-temporal dynamics of the distribution of systemically infused CTLs rlargely unknown. Noninvasive imaging of adoptively transferred CTLs using molecular-genetic reporter imaging with positron emission tomography and computed tomography (PET-CT) represents an innovative approach to understanding the long-term migratory patterns and therapeutic potential of adoptively transferred T cells. Here we report the application of repetitive PET-CT imaging with [18F]fluoro-5-ethyl-1-beta-D- arabinofuranosyluracil (18F-FEAU) in two nonhuman primates demonstrating that autologous polyclonal macaque T lymphocytes activated and transduced with a retroviral vector encoding for the sr39 mutant herpes simplex virus 1 thymidine kinase (sr39HSV1-tk) reporter gene can be detected after intravenous infusion in discrete lymphoid organs and in sites of inflammation. This study represents a proof of principle and supports the application of 18F-FEAU PET-CT imaging for monitoring the distribution of intravenously administered sr39HSV1-tk gene-transduced CTLs in humans.

AB - Adoptive transfer of antigen-specific cytotoxic T lymphocytes (CTLs) has been successfully used to treat patients with different types of cancer. However, the long-term spatial-temporal dynamics of the distribution of systemically infused CTLs rlargely unknown. Noninvasive imaging of adoptively transferred CTLs using molecular-genetic reporter imaging with positron emission tomography and computed tomography (PET-CT) represents an innovative approach to understanding the long-term migratory patterns and therapeutic potential of adoptively transferred T cells. Here we report the application of repetitive PET-CT imaging with [18F]fluoro-5-ethyl-1-beta-D- arabinofuranosyluracil (18F-FEAU) in two nonhuman primates demonstrating that autologous polyclonal macaque T lymphocytes activated and transduced with a retroviral vector encoding for the sr39 mutant herpes simplex virus 1 thymidine kinase (sr39HSV1-tk) reporter gene can be detected after intravenous infusion in discrete lymphoid organs and in sites of inflammation. This study represents a proof of principle and supports the application of 18F-FEAU PET-CT imaging for monitoring the distribution of intravenously administered sr39HSV1-tk gene-transduced CTLs in humans.

UR - http://www.scopus.com/inward/record.url?scp=70349333986&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349333986&partnerID=8YFLogxK

U2 - 10.2310/7290.2009.00022

DO - 10.2310/7290.2009.00022

M3 - Article

C2 - 19728977

AN - SCOPUS:70349333986

SN - 1535-3508

VL - 8

SP - 230

EP - 237

JO - Molecular imaging

JF - Molecular imaging

IS - 4

ER -