Resistance to mutagenesis of cells biochemically transformed by herpesvirus DNA fragments

David Trkula; Haruki Otsuka; William Plunkett

doi:10.1016/0006-291X(85)91244-6

Resistance to mutagenesis of cells biochemically transformed by herpesvirus DNA fragments

David Trkula, Haruki Otsuka, William Plunkett

Experimental Therapeutics

Research output: Contribution to journal › Article › peer-review

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Abstract

LM(TK^-) mouse fibroblast cells that were biochemically transformed to the dThd kinase-positive phenotype by restriction nuclease fragments of herpes simplex virus or marmoset herpesvirus DNA, all of which contained the virus dThd kinase coding region, or by HeLa S3 DNA were more resistant to mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine or 5-bromodeoxyuridine than were dThd kinase-positive LM and LM(TK^-) cells. Measurements of dNTP pool sizes did not reveal relative imbalances for representative cell lines under several conditions of growth.

Original language	English (US)
Pages (from-to)	1064-1071
Number of pages	8
Journal	Biochemical and biophysical research communications
Volume	133
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(85)91244-6
State	Published - Dec 31 1985

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "Resistance to mutagenesis of cells biochemically transformed by herpesvirus DNA fragments",

abstract = "LM(TK-) mouse fibroblast cells that were biochemically transformed to the dThd kinase-positive phenotype by restriction nuclease fragments of herpes simplex virus or marmoset herpesvirus DNA, all of which contained the virus dThd kinase coding region, or by HeLa S3 DNA were more resistant to mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine or 5-bromodeoxyuridine than were dThd kinase-positive LM and LM(TK-) cells. Measurements of dNTP pool sizes did not reveal relative imbalances for representative cell lines under several conditions of growth.",

author = "David Trkula and Haruki Otsuka and William Plunkett",

note = "Funding Information: ACKNOWLEDGEMENTS: We thank Dr. Kenneth Beattie initial measurements on dNTP pool sizes. This by grant No. 5-ROl-ES-02997 from the National Health Sciences.",

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T1 - Resistance to mutagenesis of cells biochemically transformed by herpesvirus DNA fragments

AU - Trkula, David

AU - Otsuka, Haruki

AU - Plunkett, William

N1 - Funding Information: ACKNOWLEDGEMENTS: We thank Dr. Kenneth Beattie initial measurements on dNTP pool sizes. This by grant No. 5-ROl-ES-02997 from the National Health Sciences.

PY - 1985/12/31

Y1 - 1985/12/31

N2 - LM(TK-) mouse fibroblast cells that were biochemically transformed to the dThd kinase-positive phenotype by restriction nuclease fragments of herpes simplex virus or marmoset herpesvirus DNA, all of which contained the virus dThd kinase coding region, or by HeLa S3 DNA were more resistant to mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine or 5-bromodeoxyuridine than were dThd kinase-positive LM and LM(TK-) cells. Measurements of dNTP pool sizes did not reveal relative imbalances for representative cell lines under several conditions of growth.

AB - LM(TK-) mouse fibroblast cells that were biochemically transformed to the dThd kinase-positive phenotype by restriction nuclease fragments of herpes simplex virus or marmoset herpesvirus DNA, all of which contained the virus dThd kinase coding region, or by HeLa S3 DNA were more resistant to mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine or 5-bromodeoxyuridine than were dThd kinase-positive LM and LM(TK-) cells. Measurements of dNTP pool sizes did not reveal relative imbalances for representative cell lines under several conditions of growth.

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Resistance to mutagenesis of cells biochemically transformed by herpesvirus DNA fragments

Abstract

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