TY - JOUR
T1 - Response of selected tumour cell populations separated from a fibrosarcoma following irradiation in situ with fast neutrons
AU - Grdina, D. J.
AU - Linde, S.
AU - Mason, K.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1978
Y1 - 1978
N2 - The authors have characterized selected populations of fibrosarcoma cell separated on density gradients of Renografin with respect to distribution in the cell cycle, clonogenic ability, and response to radiation. The latter two parameters have been observed to be related to the density of the cells, the denser populations being less clonogenic but more resistant to both low and high LET radiation. We have determined that these cells effectively repair potentially lethal damage if allowed to remain in situ following exposure to low LET radiation. This repair ability is not observed, however, following neutron irradiation. In addition, cells left in situ are relatively more sensitive to low doses of either low or high LET radiation. This difference in response is believed to be due to differences in the expression of damage by cells left in situ as compared to those assayed immediately. These data reflect the usefulness of cell separation methods in the study of solid tumour systems. Specifically, these methods reduce the heterogeneity found in the solid tumour by allowing for the isolation of subpopulations which then can be studied either individually or in relation to the entire tumour.
AB - The authors have characterized selected populations of fibrosarcoma cell separated on density gradients of Renografin with respect to distribution in the cell cycle, clonogenic ability, and response to radiation. The latter two parameters have been observed to be related to the density of the cells, the denser populations being less clonogenic but more resistant to both low and high LET radiation. We have determined that these cells effectively repair potentially lethal damage if allowed to remain in situ following exposure to low LET radiation. This repair ability is not observed, however, following neutron irradiation. In addition, cells left in situ are relatively more sensitive to low doses of either low or high LET radiation. This difference in response is believed to be due to differences in the expression of damage by cells left in situ as compared to those assayed immediately. These data reflect the usefulness of cell separation methods in the study of solid tumour systems. Specifically, these methods reduce the heterogeneity found in the solid tumour by allowing for the isolation of subpopulations which then can be studied either individually or in relation to the entire tumour.
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U2 - 10.1259/0007-1285-51-604-291
DO - 10.1259/0007-1285-51-604-291
M3 - Article
C2 - 647186
AN - SCOPUS:0017885591
SN - 0007-1285
VL - 51
SP - 291
EP - 301
JO - British Journal of Radiology
JF - British Journal of Radiology
IS - 604
ER -