TY - JOUR
T1 - Restoration of spermatogenesis in dibromochloropropane (DBCP)-treated rats by hormone suppression
AU - Meistrich, Marvin L.
AU - Wilson, Gene
AU - Porter, Karen L.
AU - Huhtaniemi, Ilpo
AU - Shetty, Gunapala
AU - Shuttlesworth, Gladis A.
PY - 2003/12
Y1 - 2003/12
N2 - The exposure of men to the nematocide dibromochloropropane (DBCP) has caused prolonged oligo- and azoospermia, which occasionally reverses spontaneously. We recently demonstrated that in testes of rats treated with a dose of DBCP sufficient to reduce the percentage of tubules producing differentiating germ cells (tubule differentiation index, TDI) to 20%, the tubules lacking differentiating cells contained type A spermatogonia. To determine whether these type A spermatogonia could be stimulated to differentiate, as had been demonstrated previously in other models of toxicant-induced sterility, we suppressed intratesticular testosterone and serum follicle stimulating hormone (FSH) levels with the GnRH agonist Lupron (leuprolide). When the GnRH agonist was given for 10 weeks starting immediately after DBCP exposure, the TDI was maintained at 94%. Even when GnRH-agonist treatment was stopped at week 10, the TDI remained between 65 and 80% 10 weeks later. Late spermatid counts aver-aged 10 × 106 per testis for the GnRH-agonist-treated rats at week 20 compared with 1.7 × 106 per testis in rats treated with only DBCP. To determine whether spermatogonial differentiation could be stimulated after the TDI had declined to below 30%, we initiated GnRH-agonist treatment 6 weeks after DBCP exposure. The GnRH treatment increased the TDI to 53% at week 16. These results indicate that, if the same principles apply to humans, suppression of testosterone may be applied to restore spermatogenesis in men rendered azoospermic by DBCP or other reproductive toxicants.
AB - The exposure of men to the nematocide dibromochloropropane (DBCP) has caused prolonged oligo- and azoospermia, which occasionally reverses spontaneously. We recently demonstrated that in testes of rats treated with a dose of DBCP sufficient to reduce the percentage of tubules producing differentiating germ cells (tubule differentiation index, TDI) to 20%, the tubules lacking differentiating cells contained type A spermatogonia. To determine whether these type A spermatogonia could be stimulated to differentiate, as had been demonstrated previously in other models of toxicant-induced sterility, we suppressed intratesticular testosterone and serum follicle stimulating hormone (FSH) levels with the GnRH agonist Lupron (leuprolide). When the GnRH agonist was given for 10 weeks starting immediately after DBCP exposure, the TDI was maintained at 94%. Even when GnRH-agonist treatment was stopped at week 10, the TDI remained between 65 and 80% 10 weeks later. Late spermatid counts aver-aged 10 × 106 per testis for the GnRH-agonist-treated rats at week 20 compared with 1.7 × 106 per testis in rats treated with only DBCP. To determine whether spermatogonial differentiation could be stimulated after the TDI had declined to below 30%, we initiated GnRH-agonist treatment 6 weeks after DBCP exposure. The GnRH treatment increased the TDI to 53% at week 16. These results indicate that, if the same principles apply to humans, suppression of testosterone may be applied to restore spermatogenesis in men rendered azoospermic by DBCP or other reproductive toxicants.
KW - Gonadotropin-releasing hormone analogs
KW - Reproductive toxicants
KW - Spermatogenia
UR - http://www.scopus.com/inward/record.url?scp=0347029996&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0347029996&partnerID=8YFLogxK
U2 - 10.1093/toxsci/kfg237
DO - 10.1093/toxsci/kfg237
M3 - Article
C2 - 14514963
AN - SCOPUS:0347029996
SN - 1096-6080
VL - 76
SP - 418
EP - 426
JO - Toxicological Sciences
JF - Toxicological Sciences
IS - 2
ER -