Abstract
AIM: To study the correlation between ITS sequence variation of Cnidium monnieri and geographical distribution and chemo-type of the plant. METHODS: Two pairs of primers Pf + Pb and P5.8S ITS1+P5.8S ITS2 were designed. The PCR products were purified and sequenced by silver staining DNA sequencing technique and ABI 310. RESULTS: Complete sequence of ITS and 5.8S rDNA, and partial sequence of 18S rDNA and 26S rDNA of Cnidium monnieri were obtained. They totaled about 700 bp. The sequences of ITS-1 and ITS-2 ranged from 210 bp to 217 bp and 219 bp to 224 bp; respectively. Pairwese sequence divergences of the samples ranged from 0% to 1.93% in ITS-1, and from 0.46% to 2.34% in ITS-2. The ITS-1 is more conservative. Phylogenetic tree based on ITS-2 sequence data (225 aligned sites) indicated that the Harbin samples, Hengshui and Dezhou samples, and Zhengzhou and Gaochun samples clustered separately. CONCLUSION: The sequence variation of ITS-2 is correlated to latitude distribution of Cnidium monnieri in China. Further studies are needed to clarify the relation between ITS sequence variation and chemo-type of Cnidium monnieri.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 56-59 |
| Number of pages | 4 |
| Journal | Yaoxue Xuebao |
| Volume | 35 |
| Issue number | 1 |
| State | Published - Jan 2000 |
| Externally published | Yes |
Keywords
- Cnidium monnieri
- Latitude
- rDNA internal transcribed spacer
ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology, Toxicology and Pharmaceutics(all)