Robust enumeration of cell subsets from tissue expression profiles

Aaron M. Newman; Chih Long Liu; Michael R. Green; Andrew J. Gentles; Weiguo Feng; Yue Xu; Chuong D. Hoang; Maximilian Diehn; Ash A. Alizadeh

doi:10.1038/nmeth.3337

Robust enumeration of cell subsets from tissue expression profiles

Aaron M. Newman, Chih Long Liu, Michael R. Green, Andrew J. Gentles, Weiguo Feng, Yue Xu, Chuong D. Hoang, Maximilian Diehn, Ash A. Alizadeh

Research output: Contribution to journal › Article › peer-review

7006 Scopus citations

Abstract

We introduce CIBERSORT, a method for characterizing cell composition of complex tissues from their gene expression profiles. When applied to enumeration of hematopoietic subsets in RNA mixtures from fresh, frozen and fixed tissues, including solid tumors, CIBERSORT outperformed other methods with respect to noise, unknown mixture content and closely related cell types. CIBERSORT should enable large-scale analysis of RNA mixtures for cellular biomarkers and therapeutic targets (http://cibersort.stanford.edu/).

Original language	English (US)
Pages (from-to)	453-457
Number of pages	5
Journal	Nature Methods
Volume	12
Issue number	5
DOIs	https://doi.org/10.1038/nmeth.3337
State	Published - Apr 29 2015
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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title = "Robust enumeration of cell subsets from tissue expression profiles",

abstract = "We introduce CIBERSORT, a method for characterizing cell composition of complex tissues from their gene expression profiles. When applied to enumeration of hematopoietic subsets in RNA mixtures from fresh, frozen and fixed tissues, including solid tumors, CIBERSORT outperformed other methods with respect to noise, unknown mixture content and closely related cell types. CIBERSORT should enable large-scale analysis of RNA mixtures for cellular biomarkers and therapeutic targets (http://cibersort.stanford.edu/).",

author = "Newman, {Aaron M.} and Liu, {Chih Long} and Green, {Michael R.} and Gentles, {Andrew J.} and Weiguo Feng and Yue Xu and Hoang, {Chuong D.} and Maximilian Diehn and Alizadeh, {Ash A.}",

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AU - Newman, Aaron M.

AU - Liu, Chih Long

AU - Green, Michael R.

AU - Gentles, Andrew J.

AU - Feng, Weiguo

AU - Xu, Yue

AU - Hoang, Chuong D.

AU - Diehn, Maximilian

AU - Alizadeh, Ash A.

PY - 2015/4/29

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AB - We introduce CIBERSORT, a method for characterizing cell composition of complex tissues from their gene expression profiles. When applied to enumeration of hematopoietic subsets in RNA mixtures from fresh, frozen and fixed tissues, including solid tumors, CIBERSORT outperformed other methods with respect to noise, unknown mixture content and closely related cell types. CIBERSORT should enable large-scale analysis of RNA mixtures for cellular biomarkers and therapeutic targets (http://cibersort.stanford.edu/).

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JO - Nature Methods

JF - Nature Methods

IS - 5

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Robust enumeration of cell subsets from tissue expression profiles

Abstract

ASJC Scopus subject areas

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