TY - JOUR
T1 - Role of apoptotic proteins in REC-2006 mediated radiation protection in hepatoma cell lines
AU - Singh, Pankaj Kumar
AU - Kumar, Raj
AU - Sharma, Ashok
AU - Arora, Rajesh
AU - Chawla, Raman
AU - Jain, Swatantra Kumar
AU - Tripathi, Rajendra Prasad
AU - Sharma, Rakesh Kumar
PY - 2011
Y1 - 2011
N2 - The present study was carried out to evaluate the role of apoptotic proteins in REC-2006-mediated radiation protection in hepatoma cell lines. REC-2006 treatment 2h before irradiation strongly inhibited the cleavage of ATM and PARP-1 in HepG2 cells. The expression of nuclear apoptosis inducing factor (AIF) was found to be more inhibited (∼17%) in HepG2 cells in REC-2006 + radiation-treated group. More inhibition (∼33%) of cytochrome c was observed in HepG2 cells upon REC-2006 treatment 2h prior irradiation. Similarly, significantly more (P<.05) inhibition of Apaf-1, caspase-9 and caspase-3 was observed in REC-2006 + radition-treated group in HepG2 cells. REC-2006 treatment restored the expression of ICAD in HepG2 cells; however, no restoration was observed in Hep3B cells. Lower nuclear to cytoplasmic CAD ratio was observed in HepG2 cells (∼0.6) as compared with Hep3B cells (∼1.2) in REC-2006 + radiation-treated group. In conclusion, REC-2006 rendered higher protection in HepG2 cells by inhibiting the expression and translocation of AIF, inhibiting the cleavage of ATM and PARP-1, restoring the expression of ICAD, inhibiting the release of cytochrome c and thus modulating the expression of Apaf-1 caspase-9 and activity of caspase-3.
AB - The present study was carried out to evaluate the role of apoptotic proteins in REC-2006-mediated radiation protection in hepatoma cell lines. REC-2006 treatment 2h before irradiation strongly inhibited the cleavage of ATM and PARP-1 in HepG2 cells. The expression of nuclear apoptosis inducing factor (AIF) was found to be more inhibited (∼17%) in HepG2 cells in REC-2006 + radiation-treated group. More inhibition (∼33%) of cytochrome c was observed in HepG2 cells upon REC-2006 treatment 2h prior irradiation. Similarly, significantly more (P<.05) inhibition of Apaf-1, caspase-9 and caspase-3 was observed in REC-2006 + radition-treated group in HepG2 cells. REC-2006 treatment restored the expression of ICAD in HepG2 cells; however, no restoration was observed in Hep3B cells. Lower nuclear to cytoplasmic CAD ratio was observed in HepG2 cells (∼0.6) as compared with Hep3B cells (∼1.2) in REC-2006 + radiation-treated group. In conclusion, REC-2006 rendered higher protection in HepG2 cells by inhibiting the expression and translocation of AIF, inhibiting the cleavage of ATM and PARP-1, restoring the expression of ICAD, inhibiting the release of cytochrome c and thus modulating the expression of Apaf-1 caspase-9 and activity of caspase-3.
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U2 - 10.1093/ecam/neq059
DO - 10.1093/ecam/neq059
M3 - Article
C2 - 21799693
AN - SCOPUS:79955096206
SN - 1741-427X
VL - 2011
JO - Evidence-based Complementary and Alternative Medicine
JF - Evidence-based Complementary and Alternative Medicine
M1 - 758326
ER -