Role of chromatin structure in the repair of DNA photoproducts in mammalian cells

Rodney S. Nairn; David L. Mitchell; Judith M. Clarkson

doi:10.1080/09553008514550251

Role of chromatin structure in the repair of DNA photoproducts in mammalian cells

Rodney S. Nairn, David L. Mitchell, Judith M. Clarkson

Epigenetics & Molecular Carcinogenesis

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Chinese hamster ovary cells were irradiated with u.v. light and chromatin was isolated and fractionated according to magnesium-solubility in low ionic strength conditions. The minor, magnesium-soluble chromatin fraction was demonstrated to be enriched by over three-fold in transcriptionally active sequences and had proteins associated with it characteristic of transcriptionally active chromatin. Using a sensitive radioimmunoassay for u.v.-induced DNA photoproducts, we have shown that there are no differences in either induction or repair of u.v. DNA photoproducts in chromatin fractions from cells irradiated at low u.v. fluence.

Original language	English (US)
Pages (from-to)	181-189
Number of pages	9
Journal	International journal of radiation biology
Volume	47
Issue number	2
DOIs	https://doi.org/10.1080/09553008514550251
State	Published - 1985

Keywords

Radio-immunoassay
Transcriptionally-active chromatin
U.v. irradiation

ASJC Scopus subject areas

Radiological and Ultrasound Technology
Radiology Nuclear Medicine and imaging

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10.1080/09553008514550251

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title = "Role of chromatin structure in the repair of DNA photoproducts in mammalian cells",

abstract = "Chinese hamster ovary cells were irradiated with u.v. light and chromatin was isolated and fractionated according to magnesium-solubility in low ionic strength conditions. The minor, magnesium-soluble chromatin fraction was demonstrated to be enriched by over three-fold in transcriptionally active sequences and had proteins associated with it characteristic of transcriptionally active chromatin. Using a sensitive radioimmunoassay for u.v.-induced DNA photoproducts, we have shown that there are no differences in either induction or repair of u.v. DNA photoproducts in chromatin fractions from cells irradiated at low u.v. fluence.",

keywords = "Radio-immunoassay, Transcriptionally-active chromatin, U.v. irradiation",

author = "Nairn, {Rodney S.} and Mitchell, {David L.} and Clarkson, {Judith M.}",

note = "Funding Information: We acknowledge Megan Phillips for excellent technical assistance, and Andrew Butler for helpful suggestions and criticisms. This work was supported by grant CA 24540 from the National Cancer Institute.",

year = "1985",

doi = "10.1080/09553008514550251",

language = "English (US)",

volume = "47",

pages = "181--189",

journal = "International journal of radiation biology",

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TY - JOUR

T1 - Role of chromatin structure in the repair of DNA photoproducts in mammalian cells

AU - Nairn, Rodney S.

AU - Mitchell, David L.

AU - Clarkson, Judith M.

N1 - Funding Information: We acknowledge Megan Phillips for excellent technical assistance, and Andrew Butler for helpful suggestions and criticisms. This work was supported by grant CA 24540 from the National Cancer Institute.

PY - 1985

Y1 - 1985

N2 - Chinese hamster ovary cells were irradiated with u.v. light and chromatin was isolated and fractionated according to magnesium-solubility in low ionic strength conditions. The minor, magnesium-soluble chromatin fraction was demonstrated to be enriched by over three-fold in transcriptionally active sequences and had proteins associated with it characteristic of transcriptionally active chromatin. Using a sensitive radioimmunoassay for u.v.-induced DNA photoproducts, we have shown that there are no differences in either induction or repair of u.v. DNA photoproducts in chromatin fractions from cells irradiated at low u.v. fluence.

AB - Chinese hamster ovary cells were irradiated with u.v. light and chromatin was isolated and fractionated according to magnesium-solubility in low ionic strength conditions. The minor, magnesium-soluble chromatin fraction was demonstrated to be enriched by over three-fold in transcriptionally active sequences and had proteins associated with it characteristic of transcriptionally active chromatin. Using a sensitive radioimmunoassay for u.v.-induced DNA photoproducts, we have shown that there are no differences in either induction or repair of u.v. DNA photoproducts in chromatin fractions from cells irradiated at low u.v. fluence.

KW - Radio-immunoassay

KW - Transcriptionally-active chromatin

KW - U.v. irradiation

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JO - International journal of radiation biology

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ER -

Role of chromatin structure in the repair of DNA photoproducts in mammalian cells

Abstract

Keywords

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