Role of the progesterone receptor (PR) in susceptibility of mouse mammary gland to 7,12-dimethylbenz[a]anthracene-induced hormone-independent preneoplastic lesions in vitro

Robert T. Chatterton; John P. Lydon; Rajendra G. Mehta; Esnar T. Mateo; Ashley Pletz; V. Craig Jordan

doi:10.1016/S0304-3835(02)00461-5

Role of the progesterone receptor (PR) in susceptibility of mouse mammary gland to 7,12-dimethylbenz[a]anthracene-induced hormone-independent preneoplastic lesions in vitro

Robert T. Chatterton, John P. Lydon, Rajendra G. Mehta, Esnar T. Mateo, Ashley Pletz, V. Craig Jordan

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Glands of wild-type (WT) and progesterone receptor knockout (PRKO) mice were exposed to 7,12-dimethylbenz[a]anthracene (DMBA) while cultured in serum-free medium containing insulin, prolactin, aldosterone, and cortisol. Glands of WT but not PRKO mice responded to DMBA with epithelial hyperplasia after 10 days in this medium. After culture without prolactin and adrenocortical hormones for an additional 14 days, hyperplastic lesions were present only in glands of WT mice. We conclude that in the absence of PR, epithelial structures are resistant to the carcinogenic action of DMBA.

Original language	English (US)
Pages (from-to)	47-52
Number of pages	6
Journal	Cancer Letters
Volume	188
Issue number	1-2
DOIs	https://doi.org/10.1016/S0304-3835(02)00461-5
State	Published - Dec 15 2002
Externally published	Yes

Keywords

Carcinogen
Gene knockout
Mammary
Progesterone receptor
Tumor

ASJC Scopus subject areas

Oncology
Cancer Research

Access to Document

10.1016/S0304-3835(02)00461-5

Cite this

@article{1a81a1de850c4a3c8deea310463ec301,

title = "Role of the progesterone receptor (PR) in susceptibility of mouse mammary gland to 7,12-dimethylbenz[a]anthracene-induced hormone-independent preneoplastic lesions in vitro",

abstract = "Glands of wild-type (WT) and progesterone receptor knockout (PRKO) mice were exposed to 7,12-dimethylbenz[a]anthracene (DMBA) while cultured in serum-free medium containing insulin, prolactin, aldosterone, and cortisol. Glands of WT but not PRKO mice responded to DMBA with epithelial hyperplasia after 10 days in this medium. After culture without prolactin and adrenocortical hormones for an additional 14 days, hyperplastic lesions were present only in glands of WT mice. We conclude that in the absence of PR, epithelial structures are resistant to the carcinogenic action of DMBA.",

keywords = "Carcinogen, Gene knockout, Mammary, Progesterone receptor, Tumor",

author = "Chatterton, {Robert T.} and Lydon, {John P.} and Mehta, {Rajendra G.} and Mateo, {Esnar T.} and Ashley Pletz and Jordan, {V. Craig}",

year = "2002",

month = dec,

day = "15",

doi = "10.1016/S0304-3835(02)00461-5",

language = "English (US)",

volume = "188",

pages = "47--52",

journal = "Cancer Letters",

issn = "0304-3835",

publisher = "Elsevier Ireland Ltd",

number = "1-2",

}

TY - JOUR

T1 - Role of the progesterone receptor (PR) in susceptibility of mouse mammary gland to 7,12-dimethylbenz[a]anthracene-induced hormone-independent preneoplastic lesions in vitro

AU - Chatterton, Robert T.

AU - Lydon, John P.

AU - Mehta, Rajendra G.

AU - Mateo, Esnar T.

AU - Pletz, Ashley

AU - Jordan, V. Craig

PY - 2002/12/15

Y1 - 2002/12/15

N2 - Glands of wild-type (WT) and progesterone receptor knockout (PRKO) mice were exposed to 7,12-dimethylbenz[a]anthracene (DMBA) while cultured in serum-free medium containing insulin, prolactin, aldosterone, and cortisol. Glands of WT but not PRKO mice responded to DMBA with epithelial hyperplasia after 10 days in this medium. After culture without prolactin and adrenocortical hormones for an additional 14 days, hyperplastic lesions were present only in glands of WT mice. We conclude that in the absence of PR, epithelial structures are resistant to the carcinogenic action of DMBA.

AB - Glands of wild-type (WT) and progesterone receptor knockout (PRKO) mice were exposed to 7,12-dimethylbenz[a]anthracene (DMBA) while cultured in serum-free medium containing insulin, prolactin, aldosterone, and cortisol. Glands of WT but not PRKO mice responded to DMBA with epithelial hyperplasia after 10 days in this medium. After culture without prolactin and adrenocortical hormones for an additional 14 days, hyperplastic lesions were present only in glands of WT mice. We conclude that in the absence of PR, epithelial structures are resistant to the carcinogenic action of DMBA.

KW - Carcinogen

KW - Gene knockout

KW - Mammary

KW - Progesterone receptor

KW - Tumor

UR - http://www.scopus.com/inward/record.url?scp=0037114345&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037114345&partnerID=8YFLogxK

U2 - 10.1016/S0304-3835(02)00461-5

DO - 10.1016/S0304-3835(02)00461-5

M3 - Article

C2 - 12406547

AN - SCOPUS:0037114345

SN - 0304-3835

VL - 188

SP - 47

EP - 52

JO - Cancer Letters

JF - Cancer Letters

IS - 1-2

ER -

Role of the progesterone receptor (PR) in susceptibility of mouse mammary gland to 7,12-dimethylbenz[a]anthracene-induced hormone-independent preneoplastic lesions in vitro

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this