Role of vasostatin-1 C-terminal region in fibroblast cell adhesion

Eleonora Dondossola; Anna Gasparri; Angela Bachi; Renato Longhi; Marie Hélène Metz-Boutigue; Bruno Tota; Karen B. Helle; Flavio Curnis; Angelo Corti

doi:10.1007/s00018-010-0319-5

Role of vasostatin-1 C-terminal region in fibroblast cell adhesion

Eleonora Dondossola, Anna Gasparri, Angela Bachi, Renato Longhi, Marie Hélène Metz-Boutigue, Bruno Tota, Karen B. Helle, Flavio Curnis, Angelo Corti

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Fibroblast adhesion can be modulated by proteins released by neuroendocrine cells and neurons, such as chromogranin A (CgA) and its N-terminal fragment vasostatin-1 (VS-1, CgA_1-78). We have investigated the mechanisms of the interaction of VS-1 with fibroblasts and of its pro-adhesive activity and have found that the proadhesive activity of VS-1 relies on its interaction with the fibroblast membrane via a phospholipid-binding amphipathic α-helix located within residues 47-66, as well as on the interaction of the adjacent C-terminal region 67-78, which is structurally similar to ezrin-radixin-moesin- binding phosphoprotein 50 (a membrane-cytoskeleton adapter protein), with other cellular components critical for the regulation of cell cytoskeleton.

Original language	English (US)
Pages (from-to)	2107-2118
Number of pages	12
Journal	Cellular and Molecular Life Sciences
Volume	67
Issue number	12
DOIs	https://doi.org/10.1007/s00018-010-0319-5
State	Published - Jun 2010
Externally published	Yes

Keywords

Cell adhesion
Chromogranin A
Cytoskeleton
Ezrin
Ezrin-radixin-moesin-binding phosphoprotein 50
Vasostatin-I

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Pharmacology
Cellular and Molecular Neuroscience
Cell Biology

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10.1007/s00018-010-0319-5

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title = "Role of vasostatin-1 C-terminal region in fibroblast cell adhesion",

abstract = "Fibroblast adhesion can be modulated by proteins released by neuroendocrine cells and neurons, such as chromogranin A (CgA) and its N-terminal fragment vasostatin-1 (VS-1, CgA1-78). We have investigated the mechanisms of the interaction of VS-1 with fibroblasts and of its pro-adhesive activity and have found that the proadhesive activity of VS-1 relies on its interaction with the fibroblast membrane via a phospholipid-binding amphipathic α-helix located within residues 47-66, as well as on the interaction of the adjacent C-terminal region 67-78, which is structurally similar to ezrin-radixin-moesin- binding phosphoprotein 50 (a membrane-cytoskeleton adapter protein), with other cellular components critical for the regulation of cell cytoskeleton.",

keywords = "Cell adhesion, Chromogranin A, Cytoskeleton, Ezrin, Ezrin-radixin-moesin-binding phosphoprotein 50, Vasostatin-I",

author = "Eleonora Dondossola and Anna Gasparri and Angela Bachi and Renato Longhi and Metz-Boutigue, {Marie H{\'e}l{\`e}ne} and Bruno Tota and Helle, {Karen B.} and Flavio Curnis and Angelo Corti",

note = "Funding Information: This work was supported by Associazione Italiana per la Ricerca sul Cancro (AIRC) and Ministero della Sanit{\`a} of Italy. We thank Alessandra Boletta for helpful discussion.",

year = "2010",

month = jun,

doi = "10.1007/s00018-010-0319-5",

language = "English (US)",

volume = "67",

pages = "2107--2118",

journal = "Cellular and Molecular Life Sciences",

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T1 - Role of vasostatin-1 C-terminal region in fibroblast cell adhesion

AU - Dondossola, Eleonora

AU - Gasparri, Anna

AU - Bachi, Angela

AU - Longhi, Renato

AU - Metz-Boutigue, Marie Hélène

AU - Tota, Bruno

AU - Helle, Karen B.

AU - Curnis, Flavio

AU - Corti, Angelo

N1 - Funding Information: This work was supported by Associazione Italiana per la Ricerca sul Cancro (AIRC) and Ministero della Sanità of Italy. We thank Alessandra Boletta for helpful discussion.

PY - 2010/6

Y1 - 2010/6

N2 - Fibroblast adhesion can be modulated by proteins released by neuroendocrine cells and neurons, such as chromogranin A (CgA) and its N-terminal fragment vasostatin-1 (VS-1, CgA1-78). We have investigated the mechanisms of the interaction of VS-1 with fibroblasts and of its pro-adhesive activity and have found that the proadhesive activity of VS-1 relies on its interaction with the fibroblast membrane via a phospholipid-binding amphipathic α-helix located within residues 47-66, as well as on the interaction of the adjacent C-terminal region 67-78, which is structurally similar to ezrin-radixin-moesin- binding phosphoprotein 50 (a membrane-cytoskeleton adapter protein), with other cellular components critical for the regulation of cell cytoskeleton.

AB - Fibroblast adhesion can be modulated by proteins released by neuroendocrine cells and neurons, such as chromogranin A (CgA) and its N-terminal fragment vasostatin-1 (VS-1, CgA1-78). We have investigated the mechanisms of the interaction of VS-1 with fibroblasts and of its pro-adhesive activity and have found that the proadhesive activity of VS-1 relies on its interaction with the fibroblast membrane via a phospholipid-binding amphipathic α-helix located within residues 47-66, as well as on the interaction of the adjacent C-terminal region 67-78, which is structurally similar to ezrin-radixin-moesin- binding phosphoprotein 50 (a membrane-cytoskeleton adapter protein), with other cellular components critical for the regulation of cell cytoskeleton.

KW - Cell adhesion

KW - Chromogranin A

KW - Cytoskeleton

KW - Ezrin

KW - Ezrin-radixin-moesin-binding phosphoprotein 50

KW - Vasostatin-I

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JO - Cellular and Molecular Life Sciences

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ER -

Role of vasostatin-1 C-terminal region in fibroblast cell adhesion

Abstract

Keywords

ASJC Scopus subject areas

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