RSC-Associated Subnucleosomes Define MNase-Sensitive Promoters in Yeast

Sandipan Brahma; Steven Henikoff

doi:10.1016/j.molcel.2018.10.046

RSC-Associated Subnucleosomes Define MNase-Sensitive Promoters in Yeast

Sandipan Brahma, Steven Henikoff

Epigenetics & Molecular Carcinogenesis

Research output: Contribution to journal › Article › peer-review

75 Scopus citations

Abstract

Brahma and Henikoff resolve the composition of MNase-sensitive particles at yeast promoters by showing that these are RSC- and GRF-associated, partially unwrapped nucleosomal intermediates. For this, Brahma and Henikoff combined a targeted chromatin cleavage and release approach with native ChIP, which would be widely applicable for genomic profiling of chromatin-factor co-occupancies.

Original language	English (US)
Pages (from-to)	238-249.e3
Journal	Molecular cell
Volume	73
Issue number	2
DOIs	https://doi.org/10.1016/j.molcel.2018.10.046
State	Published - Jan 17 2019

Keywords

CUT&RUN
fragile nucleosome
general regulatory factor
nucleosome dynamics
nucleosome-depleted region

ASJC Scopus subject areas

Molecular Biology
Cell Biology

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10.1016/j.molcel.2018.10.046

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title = "RSC-Associated Subnucleosomes Define MNase-Sensitive Promoters in Yeast",

abstract = "Brahma and Henikoff resolve the composition of MNase-sensitive particles at yeast promoters by showing that these are RSC- and GRF-associated, partially unwrapped nucleosomal intermediates. For this, Brahma and Henikoff combined a targeted chromatin cleavage and release approach with native ChIP, which would be widely applicable for genomic profiling of chromatin-factor co-occupancies.",

keywords = "CUT&RUN, fragile nucleosome, general regulatory factor, nucleosome dynamics, nucleosome-depleted region",

author = "Sandipan Brahma and Steven Henikoff",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",

year = "2019",

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language = "English (US)",

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AU - Brahma, Sandipan

AU - Henikoff, Steven

PY - 2019/1/17

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N2 - Brahma and Henikoff resolve the composition of MNase-sensitive particles at yeast promoters by showing that these are RSC- and GRF-associated, partially unwrapped nucleosomal intermediates. For this, Brahma and Henikoff combined a targeted chromatin cleavage and release approach with native ChIP, which would be widely applicable for genomic profiling of chromatin-factor co-occupancies.

AB - Brahma and Henikoff resolve the composition of MNase-sensitive particles at yeast promoters by showing that these are RSC- and GRF-associated, partially unwrapped nucleosomal intermediates. For this, Brahma and Henikoff combined a targeted chromatin cleavage and release approach with native ChIP, which would be widely applicable for genomic profiling of chromatin-factor co-occupancies.

KW - CUT&RUN

KW - fragile nucleosome

KW - general regulatory factor

KW - nucleosome dynamics

KW - nucleosome-depleted region

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RSC-Associated Subnucleosomes Define MNase-Sensitive Promoters in Yeast

Abstract

Keywords

ASJC Scopus subject areas

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