TY - JOUR
T1 - Salt-induced redox-independent phosphorylation of light harvesting chlorophyll a/b proteins in Dunaliella salina thylakoid membranes
AU - Liu, Xian De
AU - Shen, Yun Gang
N1 - Funding Information:
The authors wish to thank Professor Jia-Mian Wei for critical comments on the manuscript. This work was supported by the State Key Basic Development Plan Program.
PY - 2005/2/17
Y1 - 2005/2/17
N2 - This study investigated the regulation of the major light harvesting chlorophyll a/b protein (LHCII) phosphorylation in Dunaliella salina thylakoid membranes. We found that both light and NaCl could induce LHCII phosphorylation in D. salina thylakoid membranes. Treatments with oxidants (ferredoxin and NADP) or photosynthetic electron flow inhibitors (DCMU, DBMIB, and stigmatellin) inhibited LHCII phosphorylation induced by light but not that induced by NaCl. Furthermore, neither addition of CuCl2, an inhibitor of cytochrome b6f complex reduction, nor oxidizing treatment with ferricyanide inhibited light- or NaCl-induced LHCII phosphorylation, and both salts even induced LHCII phosphorylation in dark-adapted D. salina thylakoid membranes as other salts did. Together, these results indicate that the redox state of the cytochrome b6f complex is likely involved in light- but not salt-induced LHCII phosphorylation in D. salina thylakoid membranes.
AB - This study investigated the regulation of the major light harvesting chlorophyll a/b protein (LHCII) phosphorylation in Dunaliella salina thylakoid membranes. We found that both light and NaCl could induce LHCII phosphorylation in D. salina thylakoid membranes. Treatments with oxidants (ferredoxin and NADP) or photosynthetic electron flow inhibitors (DCMU, DBMIB, and stigmatellin) inhibited LHCII phosphorylation induced by light but not that induced by NaCl. Furthermore, neither addition of CuCl2, an inhibitor of cytochrome b6f complex reduction, nor oxidizing treatment with ferricyanide inhibited light- or NaCl-induced LHCII phosphorylation, and both salts even induced LHCII phosphorylation in dark-adapted D. salina thylakoid membranes as other salts did. Together, these results indicate that the redox state of the cytochrome b6f complex is likely involved in light- but not salt-induced LHCII phosphorylation in D. salina thylakoid membranes.
KW - Cytochrome bf complex
KW - Dunaliella salina
KW - LHCII phosphorylation
KW - NaCl
KW - Redox state
KW - Thylakoid membrane
UR - http://www.scopus.com/inward/record.url?scp=13444261979&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=13444261979&partnerID=8YFLogxK
U2 - 10.1016/j.bbabio.2004.11.003
DO - 10.1016/j.bbabio.2004.11.003
M3 - Article
C2 - 15694349
AN - SCOPUS:13444261979
SN - 0005-2728
VL - 1706
SP - 215
EP - 219
JO - Biochimica et Biophysica Acta - Bioenergetics
JF - Biochimica et Biophysica Acta - Bioenergetics
IS - 3
ER -