(-)Schisandrin B is more potent than its enantiomer in enhancing cellular glutathione and heat shock protein production as well as protecting against oxidant injury in H9c2 cardiomyocytes

Po Yee Chiu; Hoi Yan Leung; Michel K.T. Poon; Duncan H.F. Mak; Kam Ming Ko

doi:10.1007/s11010-006-9163-1

(-)Schisandrin B is more potent than its enantiomer in enhancing cellular glutathione and heat shock protein production as well as protecting against oxidant injury in H9c2 cardiomyocytes

Po Yee Chiu, Hoi Yan Leung, Michel K.T. Poon, Duncan H.F. Mak, Kam Ming Ko

Leukemia

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21 Scopus citations

Abstract

Effects of schisandrin B enantiomers ((+)Sch B and (-)Sch B) treatment on cellular reduced glutathione (GSH) level and heat shock protein (Hsp)25/70 production were investigated in H9c2 cardiomyocytes. (+)Sch B and (-)Sch B at 6.25 μM produced a time-dependent and biphasic change in cellular GSH level and Hsp25/70 production, with the stimulatory effect of (-)Sch B being more potent. The GSH- and Hsp-enhancing effects were accompanied by a parallel cytoprotection against xanthine oxidase/ xanthine-induced toxicity, with the biphasic time course of (+)Sch B- or (-)Sch B-induced protection being superimposed with that of the increase in GSH level but not Hsp25/70 production. The results indicate that (-)Sch B produces more potent enhancing effects on cellular GSH and Hsp production as well as protection against oxidative injury than (+)Sch B in cardiomyocytes.

Original language	English (US)
Pages (from-to)	185-191
Number of pages	7
Journal	Molecular and Cellular Biochemistry
Volume	289
Issue number	1-2
DOIs	https://doi.org/10.1007/s11010-006-9163-1
State	Published - Sep 2006

Keywords

Cardiomyocytes
Enantiomers
Glutathione
H9c2
Heat shock protein
Schisandrin B

ASJC Scopus subject areas

Molecular Biology
Clinical Biochemistry
Cell Biology

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10.1007/s11010-006-9163-1

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(-)Schisandrin B is more potent than its enantiomer in enhancing cellular glutathione and heat shock protein production as well as protecting against oxidant injury in H9c2 cardiomyocytes. / Chiu, Po Yee; Leung, Hoi Yan; Poon, Michel K.T. et al.
In: Molecular and Cellular Biochemistry, Vol. 289, No. 1-2, 09.2006, p. 185-191.

Research output: Contribution to journal › Article › peer-review

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abstract = "Effects of schisandrin B enantiomers ((+)Sch B and (-)Sch B) treatment on cellular reduced glutathione (GSH) level and heat shock protein (Hsp)25/70 production were investigated in H9c2 cardiomyocytes. (+)Sch B and (-)Sch B at 6.25 μM produced a time-dependent and biphasic change in cellular GSH level and Hsp25/70 production, with the stimulatory effect of (-)Sch B being more potent. The GSH- and Hsp-enhancing effects were accompanied by a parallel cytoprotection against xanthine oxidase/ xanthine-induced toxicity, with the biphasic time course of (+)Sch B- or (-)Sch B-induced protection being superimposed with that of the increase in GSH level but not Hsp25/70 production. The results indicate that (-)Sch B produces more potent enhancing effects on cellular GSH and Hsp production as well as protection against oxidative injury than (+)Sch B in cardiomyocytes.",

keywords = "Cardiomyocytes, Enantiomers, Glutathione, H9c2, Heat shock protein, Schisandrin B",

author = "Chiu, {Po Yee} and Leung, {Hoi Yan} and Poon, {Michel K.T.} and Mak, {Duncan H.F.} and Ko, {Kam Ming}",

note = "Funding Information: This work was supported by the grants HKUST6130/02M from the Research Grants Council, Hong Kong.",

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AU - Chiu, Po Yee

AU - Leung, Hoi Yan

AU - Poon, Michel K.T.

AU - Mak, Duncan H.F.

AU - Ko, Kam Ming

N1 - Funding Information: This work was supported by the grants HKUST6130/02M from the Research Grants Council, Hong Kong.

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N2 - Effects of schisandrin B enantiomers ((+)Sch B and (-)Sch B) treatment on cellular reduced glutathione (GSH) level and heat shock protein (Hsp)25/70 production were investigated in H9c2 cardiomyocytes. (+)Sch B and (-)Sch B at 6.25 μM produced a time-dependent and biphasic change in cellular GSH level and Hsp25/70 production, with the stimulatory effect of (-)Sch B being more potent. The GSH- and Hsp-enhancing effects were accompanied by a parallel cytoprotection against xanthine oxidase/ xanthine-induced toxicity, with the biphasic time course of (+)Sch B- or (-)Sch B-induced protection being superimposed with that of the increase in GSH level but not Hsp25/70 production. The results indicate that (-)Sch B produces more potent enhancing effects on cellular GSH and Hsp production as well as protection against oxidative injury than (+)Sch B in cardiomyocytes.

AB - Effects of schisandrin B enantiomers ((+)Sch B and (-)Sch B) treatment on cellular reduced glutathione (GSH) level and heat shock protein (Hsp)25/70 production were investigated in H9c2 cardiomyocytes. (+)Sch B and (-)Sch B at 6.25 μM produced a time-dependent and biphasic change in cellular GSH level and Hsp25/70 production, with the stimulatory effect of (-)Sch B being more potent. The GSH- and Hsp-enhancing effects were accompanied by a parallel cytoprotection against xanthine oxidase/ xanthine-induced toxicity, with the biphasic time course of (+)Sch B- or (-)Sch B-induced protection being superimposed with that of the increase in GSH level but not Hsp25/70 production. The results indicate that (-)Sch B produces more potent enhancing effects on cellular GSH and Hsp production as well as protection against oxidative injury than (+)Sch B in cardiomyocytes.

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KW - Enantiomers

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KW - H9c2

KW - Heat shock protein

KW - Schisandrin B

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(-)Schisandrin B is more potent than its enantiomer in enhancing cellular glutathione and heat shock protein production as well as protecting against oxidant injury in H9c2 cardiomyocytes

Abstract

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ASJC Scopus subject areas

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