Screening and identification of hypoxic proteins by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry

Gongyi Shi; Yijun Chen; Albert Koong

Screening and identification of hypoxic proteins by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry

Gongyi Shi, Yijun Chen, Albert Koong

Image Guided Cancer Therapy Research Program

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The screening and identification of hypoxic proteins by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry was discussed. The head & neck squamous cell carcinoma cell lines was exposed to 24 hours of hypoxia and compared with a normoxic control. These cells were lysed and subject to subcellular fractionation through a series of differential centrifugation steps. The tryptic peptide spectra were generated on a Voyager-DE PRO MALDI-TOF-MS. The endoplasmic reticulum (ER) was the primary site for secreted protein and membrane protein biogenesis, and have stress response to various environmental changes.

Original language	English (US)
Title of host publication	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	223-224
Number of pages	2
State	Published - Dec 1 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

ASJC Scopus subject areas

Spectroscopy

Cite this

@inproceedings{cadb430e927946eabce8cab743d686aa,

title = "Screening and identification of hypoxic proteins by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry",

abstract = "The screening and identification of hypoxic proteins by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry was discussed. The head & neck squamous cell carcinoma cell lines was exposed to 24 hours of hypoxia and compared with a normoxic control. These cells were lysed and subject to subcellular fractionation through a series of differential centrifugation steps. The tryptic peptide spectra were generated on a Voyager-DE PRO MALDI-TOF-MS. The endoplasmic reticulum (ER) was the primary site for secreted protein and membrane protein biogenesis, and have stress response to various environmental changes.",

author = "Gongyi Shi and Yijun Chen and Albert Koong",

year = "2002",

month = dec,

day = "1",

language = "English (US)",

pages = "223--224",

booktitle = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - GEN

T1 - Screening and identification of hypoxic proteins by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry

AU - Shi, Gongyi

AU - Chen, Yijun

AU - Koong, Albert

PY - 2002/12/1

Y1 - 2002/12/1

N2 - The screening and identification of hypoxic proteins by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry was discussed. The head & neck squamous cell carcinoma cell lines was exposed to 24 hours of hypoxia and compared with a normoxic control. These cells were lysed and subject to subcellular fractionation through a series of differential centrifugation steps. The tryptic peptide spectra were generated on a Voyager-DE PRO MALDI-TOF-MS. The endoplasmic reticulum (ER) was the primary site for secreted protein and membrane protein biogenesis, and have stress response to various environmental changes.

AB - The screening and identification of hypoxic proteins by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry was discussed. The head & neck squamous cell carcinoma cell lines was exposed to 24 hours of hypoxia and compared with a normoxic control. These cells were lysed and subject to subcellular fractionation through a series of differential centrifugation steps. The tryptic peptide spectra were generated on a Voyager-DE PRO MALDI-TOF-MS. The endoplasmic reticulum (ER) was the primary site for secreted protein and membrane protein biogenesis, and have stress response to various environmental changes.

UR - http://www.scopus.com/inward/record.url?scp=2542460412&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2542460412&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:2542460412

SP - 223

EP - 224

BT - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Screening and identification of hypoxic proteins by two-dimensional polyacrylamide gel electrophoresis and mass spectrometry

Abstract

Conference

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this