Secretory expression of a single-chain insulin precursor in yeast and its conversion into human insulin.

Y. Zhang; H. Hu; R. Cai; Y. Feng; S. Zhu; Q. He; Y. Tang; M. Xu; Y. Xu; X. Zhang; B. Liu; Z. Liang

Secretory expression of a single-chain insulin precursor in yeast and its conversion into human insulin.

Y. Zhang, H. Hu, R. Cai, Y. Feng, S. Zhu, Q. He, Y. Tang, M. Xu, Y. Xu, X. Zhang, B. Liu, Z. Liang

Gynecological Oncology & Reproductive Medicine

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

A synthetic single-chain porcine insulin precursor (PIP) gene and an alpha-mating factor leader sequence (alpha MFL) gene obtained by the PCR method are inserted between the promoter and 3'-terminating sequence of the alcohol dehydrogenase gene ADH1 in plasmid pVT102-U to form plasmid pVT102-U/alpha MFL-PIP. The single-chain insulin precursor is expressed and secreted to the culture medium by Saccharomyces cerevisiae transformed by pVT102-U/alpha MFL-PIP. The precursor is purified and converted into human insulin by tryptic transpeptidation. The purified human insulin is fully active and can be crystallized. The overall yield of human insulin is 25 mg per liter of culture medium.

Original language	English (US)
Pages (from-to)	225-233
Number of pages	9
Journal	Science in China. Series C, Life sciences / Chinese Academy of Sciences
Volume	39
Issue number	3
State	Published - Jun 1 1996

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Environmental Science
General Agricultural and Biological Sciences

Cite this

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abstract = "A synthetic single-chain porcine insulin precursor (PIP) gene and an alpha-mating factor leader sequence (alpha MFL) gene obtained by the PCR method are inserted between the promoter and 3'-terminating sequence of the alcohol dehydrogenase gene ADH1 in plasmid pVT102-U to form plasmid pVT102-U/alpha MFL-PIP. The single-chain insulin precursor is expressed and secreted to the culture medium by Saccharomyces cerevisiae transformed by pVT102-U/alpha MFL-PIP. The precursor is purified and converted into human insulin by tryptic transpeptidation. The purified human insulin is fully active and can be crystallized. The overall yield of human insulin is 25 mg per liter of culture medium.",

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AU - Zhang, Y.

AU - Hu, H.

AU - Cai, R.

AU - Feng, Y.

AU - Zhu, S.

AU - He, Q.

AU - Tang, Y.

AU - Xu, M.

AU - Xu, Y.

AU - Zhang, X.

AU - Liu, B.

AU - Liang, Z.

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Secretory expression of a single-chain insulin precursor in yeast and its conversion into human insulin.

Abstract

ASJC Scopus subject areas

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