TY - JOUR
T1 - Separation of cytotoxic leukocyte populations of human peripheral blood and colostrum on PVP-silica (Percoll®) density gradients
AU - Dvonch Steinmetz, Karen
AU - Kohl, Steve
AU - Richie, Ellen R.
N1 - Funding Information:
This study was supported by Young Investigator Grant No. AI14450 and NIH Grant PO lHD13021.
PY - 1981/4/29
Y1 - 1981/4/29
N2 - PVP-silica (Percoll) did not inhibit antibody-dependent cellular cytotoxicity in an 18 h 51Cr-release assay. By the use of a simple discontinuous density gradient centrifugation technique, leukocytes from peripheral blood and colostrum were separated into morphologic and cytotoxic functional populations. The major cytotoxic effector cells in colostrum are lipid-free macrophages and probably a small lymphocyte subpopulation.
AB - PVP-silica (Percoll) did not inhibit antibody-dependent cellular cytotoxicity in an 18 h 51Cr-release assay. By the use of a simple discontinuous density gradient centrifugation technique, leukocytes from peripheral blood and colostrum were separated into morphologic and cytotoxic functional populations. The major cytotoxic effector cells in colostrum are lipid-free macrophages and probably a small lymphocyte subpopulation.
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U2 - 10.1016/0022-1759(81)90145-9
DO - 10.1016/0022-1759(81)90145-9
M3 - Article
C2 - 6263977
AN - SCOPUS:0019464607
SN - 0022-1759
VL - 42
SP - 157
EP - 170
JO - Journal of Immunological Methods
JF - Journal of Immunological Methods
IS - 2
ER -