TY - JOUR
T1 - Separation of human breast cancer cells from blood by differential dielectric affinity
AU - Becker, Frederick F.
AU - Wang, Xiao Bo
AU - Huang, Ying
AU - Pethig, Ronald
AU - Vykoukal, Jody
AU - Gascoyne, Peter R.C.
PY - 1995/1/31
Y1 - 1995/1/31
N2 - Electrorotation measurements were used to demonstrate that the dielectric properties of the metastatic human breast cancer cell line MDA231 were significantly different from those of erythrocytes and T lymphocytes. These dielectric differences were exploited to separate the cancer cells from normal blood cells by appropriately balancing the hydrodynamic and dielectrophoretic forces acting on the cells within a dielectric affinity column containing a microelectrode array. The operational criteria for successful particle separation in such a column are analyzed and our findings indicate that the dielectric affinity technique may prove useful m a wide variety of cell separation and characterization applications.
AB - Electrorotation measurements were used to demonstrate that the dielectric properties of the metastatic human breast cancer cell line MDA231 were significantly different from those of erythrocytes and T lymphocytes. These dielectric differences were exploited to separate the cancer cells from normal blood cells by appropriately balancing the hydrodynamic and dielectrophoretic forces acting on the cells within a dielectric affinity column containing a microelectrode array. The operational criteria for successful particle separation in such a column are analyzed and our findings indicate that the dielectric affinity technique may prove useful m a wide variety of cell separation and characterization applications.
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U2 - 10.1073/pnas.92.3.860
DO - 10.1073/pnas.92.3.860
M3 - Article
C2 - 7846067
AN - SCOPUS:0028799532
SN - 0027-8424
VL - 92
SP - 860
EP - 864
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 3
ER -