Separation of isolated Chinese hamster metaphase chromosomes into three size-groups

John Mendelsohn; Dorothy E. Moore; Norman P. Salzman

doi:10.1016/0022-2836(68)90148-4

Separation of isolated Chinese hamster metaphase chromosomes into three size-groups

John Mendelsohn, Dorothy E. Moore, Norman P. Salzman

Experimental Therapeutics

Research output: Contribution to journal › Article › peer-review

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Abstract

A method is described for the isolation of metaphase Chinese hamster chromosomes and their separation into three size-groups. DNA extracted from fractionated chromosomes does not show heterogeneity in thermal denaturation temperature or buoyant density. The separation technique has been used to demonstrate a high rate of DNA synthesis in small hamster chromosomes during late S phase.

Original language	English (US)
Pages (from-to)	101-104,IN1,105-112
Journal	Journal of Molecular Biology
Volume	32
Issue number	1
DOIs	https://doi.org/10.1016/0022-2836(68)90148-4
State	Published - Feb 28 1968

ASJC Scopus subject areas

Structural Biology
Molecular Biology

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10.1016/0022-2836(68)90148-4

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title = "Separation of isolated Chinese hamster metaphase chromosomes into three size-groups",

abstract = "A method is described for the isolation of metaphase Chinese hamster chromosomes and their separation into three size-groups. DNA extracted from fractionated chromosomes does not show heterogeneity in thermal denaturation temperature or buoyant density. The separation technique has been used to demonstrate a high rate of DNA synthesis in small hamster chromosomes during late S phase.",

author = "John Mendelsohn and Moore, {Dorothy E.} and Salzman, {Norman P.}",

year = "1968",

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AU - Salzman, Norman P.

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N2 - A method is described for the isolation of metaphase Chinese hamster chromosomes and their separation into three size-groups. DNA extracted from fractionated chromosomes does not show heterogeneity in thermal denaturation temperature or buoyant density. The separation technique has been used to demonstrate a high rate of DNA synthesis in small hamster chromosomes during late S phase.

AB - A method is described for the isolation of metaphase Chinese hamster chromosomes and their separation into three size-groups. DNA extracted from fractionated chromosomes does not show heterogeneity in thermal denaturation temperature or buoyant density. The separation technique has been used to demonstrate a high rate of DNA synthesis in small hamster chromosomes during late S phase.

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Separation of isolated Chinese hamster metaphase chromosomes into three size-groups

Abstract

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